{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:11:36Z","timestamp":1790413896376,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,6,28]],"date-time":"2008-06-28T00:00:00Z","timestamp":1214611200000},"content-version":"vor","delay-in-days":2339,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p><jats:bold>Summary.<\/jats:bold> Differentiation of B lymphocytes into plasma cells is regulated by the interaction of distinct transcription factors (TFs) which activate gene expression in a lineage\u2010 and stage\u2010specific pattern. Using reverse transcription polymerase chain reaction, we studied the expression of five TFs (octamer binding factor oct\u20102, ets family members PU.1 and Spi\u2010B, pax gene family member BSAP, and Blimp\u20101) in (1) human cell lines with a plasma cell phenotype, (2) primary malignant plasma cells [obtained from patients with plasma cell leukaemia (PCL) and multiple myeloma], and (3) normal human plasma cells generated <jats:italic>in vitro<\/jats:italic> or isolated from normal bone marrows. The expression pattern was compared with TFs expressed by normal CD19<jats:sup>+<\/jats:sup> B lymphocytes and by B cells from chronic lymphocytic leukaemia patients. Our results showed that plasma cells expressed a restricted set of TFs compared with CD19<jats:sup>+<\/jats:sup> B lymphocytes, with continued expression of Spi\u2010B and oct\u20102, increased Blimp\u20101 expression, and downregulation of BSAP and PU.1. Cells from PCL lost Spi\u2010B and PU.1 expression completely and expressed only oct\u20102 and Blimp\u20101, and thus resembled plasma cell lines. Human plasma cell differentiation therefore seems to be positively regulated by Blimp\u20101; whether this TF has any oncogenic potential will have to be analysed in future studies.<\/jats:p>","DOI":"10.1046\/j.1365-2141.2002.03271.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T06:42:11Z","timestamp":1047364931000},"page":"429-435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Expression of transcription factors Pu.1, Spi\u2010B, Blimp\u20101, BSAP and oct\u20102 in normal human plasma cells and in multiple myeloma cells"],"prefix":"10.1111","volume":"116","author":[{"given":"Monika","family":"Nagy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Chapuis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Matthes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,6,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60887-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1987.9999"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.7.4.570"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1182\/blood.V94.4.1283","article-title":"A critical role for PU.1 in homing and long\u2010term engraftment by hematopoietic stem cells in the bone 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