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This method has been validated in a cohort of 144 patients with FVIII\/VWF ratios\u2003&lt;\u20030\u00b76 using a plasma control mixture representative of intermediate VWF:FVIIIB. In total, 15 patients were diagnosed with markedly decreased VWF:FVIIIB and five patients were shown to have moderately decreased VWF:FVIIIB. Specific type 2N mutations were identified in all these patients.<\/jats:p>","DOI":"10.1046\/j.1365-2141.2002.03488.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T06:45:28Z","timestamp":1047365128000},"page":"716-718","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Large experience with a factor VIII binding assay of plasma von Willebrand factor using commercial reagents"],"prefix":"10.1111","volume":"117","author":[{"given":"Claudine","family":"Caron","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudine","family":"Mazurier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jenny","family":"Goudemand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,5,19]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/109.3.347"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1651576"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830400105"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1055\/s-0038-1650567","article-title":"Factor VIII binding assay of von Willebrand factor and the diagnosis of type 2N von Willebrand disease: results of an international survey. 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