{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T07:31:23Z","timestamp":1781249483168,"version":"3.54.1"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>The breaking strength of colonic anastomoses declines after operation to a minimum at days 3\u20134, with a subsequent risk of anastomotic dehiscence. The mechanism is thought to be collagen degradation by matrix metalloproteinases (MMPs). This study examined the pathogenic role of MMPs on the mechanical strength of colonic anastomoses by giving the synthetic broad-spectrum MMP inhibitor BB-1101 systemically.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Forty-eight male Sprague\u2013Dawley rats were treated daily for 7 days with BB-1101 30 mg\/kg or vehicle alone (control) starting 2 days before operation. The breaking strength of standardized left-sided colonic anastomoses was measured on postoperative days 1, 3 and 7.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Serum BB-1101 levels were increased at 100 nmol\/l in BB-1101-treated rats. The anastomotic breaking strength was 48 per cent higher (P = 0\u00b702) in BB-1101-treated animals compared with controls on postoperative day 3. Neither collagen accumulation nor infiltration of neutrophils in the anastomotic area was influenced by BB-1101 treatment. Net deposition of new collagen in subcutaneous sponges was unaffected by the BB-1101.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>The enhanced breaking strength of colonic anastomoses during the critical early postoperative phase found after administration of a broad-spectrum MMP inhibitor implies that MMPs might increase the risk of anastomotic dehiscence.<\/jats:p>\n                  <jats:p>Presented in part to the third joint meeting of the European Tissue Repair Society and the Wound Healing Society in Bordeaux, France, 24\u201328 August 1999, and published in abstract form in Wound Repair Regen 1999; 7: A321<\/jats:p>\n               <\/jats:sec>","DOI":"10.1046\/j.1365-2168.2001.01649.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T07:21:07Z","timestamp":1047367267000},"page":"228-234","source":"Crossref","is-referenced-by-count":72,"title":["Inhibition of matrix metalloproteinases enhances breaking strength of colonic anastomoses in an experimental model"],"prefix":"10.1093","volume":"88","author":[{"given":"I","family":"Syk","sequence":"first","affiliation":[{"name":"Department of Surgery, Malm\u00f6 University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M S","family":"\u00c5gren","sequence":"additional","affiliation":[{"name":"Copenhagen Wound Healing Centre, Bispebjerg Hospital , Copenhagen,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D","family":"Adawi","sequence":"additional","affiliation":[{"name":"Department of Surgery, Malm\u00f6 University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Jeppsson","sequence":"additional","affiliation":[{"name":"Department of Surgery, Malm\u00f6 University Hospital, Lund University , Malm\u00f6,","place":["Sweden"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,2,1]]},"reference":[{"key":"2024093006063257100_bib1","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1136\/bmj.281.6237.411","article-title":"Anastomotic integrity after operations for large-bowel cancer: a multicentre study","volume":"281","author":"Fielding","year":"1980","journal-title":"BMJ"},{"key":"2024093006063257100_bib2","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1002\/bjs.1800810850","article-title":"Leakage from stapled low anastomosis after total mesorectal excision for carcinoma of the rectum","volume":"81","author":"Karanjia","year":"1994","journal-title":"Br J Surg"},{"key":"2024093006063257100_bib3","first-page":"293","article-title":"Anastomotic leakage after anterior resection of the rectum","volume":"160","author":"Pakkastie","year":"1994","journal-title":"Eur J Surg"},{"key":"2024093006063257100_bib4","doi-asserted-by":"crossref","first-page":"597","DOI":"10.1016\/S0002-9610(88)80417-3","article-title":"Analysis of techniques and results in 347 consecutive colon anastomoses","volume":"155","author":"Curley","year":"1988","journal-title":"Am J Surg"},{"key":"2024093006063257100_bib5","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1046\/j.1365-2168.1998.00615.x","article-title":"Risk factors for anastomotic leakage after resection of rectal cancer","volume":"85","author":"Rullier","year":"1998","journal-title":"Br J Surg"},{"key":"2024093006063257100_bib6","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1001\/archsurg.1991.01410350117019","article-title":"Neutrophil-dependent decrease in early wound margin strength","volume":"126","author":"Jonsson","year":"1991","journal-title":"Arch Surg"},{"key":"2024093006063257100_bib7","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1006\/jsre.1996.0367","article-title":"Collagen deposition and mechanical strength of colon anastomoses and skin incisional wounds of rats","volume":"66","author":"Oxlund","year":"1996","journal-title":"J Surg Res"},{"key":"2024093006063257100_bib8","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1006\/jsre.1999.5765","article-title":"Protease inhibitor reduces loss of tensile strength in rat anastomosis with peritonitis","volume":"88","author":"Tani","year":"2000","journal-title":"J Surg Res"},{"key":"2024093006063257100_bib9","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/BF00341294","article-title":"Collagenolytic activity in experimental intestinal anastomoses. Differences between small and large bowel and evidence for the presence of collagenase","volume":"7","author":"van der Stappen","year":"1992","journal-title":"Int J Colorectal Dis"},{"key":"2024093006063257100_bib10","doi-asserted-by":"crossref","first-page":"962","DOI":"10.1007\/BF02051206","article-title":"Role of matrix metalloproteinases in healing of colon anastomosis","volume":"40","author":"Savage","year":"1997","journal-title":"Dis Colon Rectum"},{"key":"2024093006063257100_bib11","doi-asserted-by":"crossref","first-page":"354","DOI":"10.2307\/3579344","article-title":"Intra-operative irradiation prolongs the presence of matrix metalloproteinase activity in large bowel anastomoses of the rat","volume":"147","author":"Seifert","year":"1997","journal-title":"Radiat Res"},{"key":"2024093006063257100_bib12","first-page":"1005","article-title":"Distinct expression profiles of stromelysin-2 (MMP-10), collagenase-3 (MMP-13), macrophage metalloelastase (MMP-12), and tissue inhibitor of metalloproteinases-3 (TIMP-3) in intestinal ulcerations","volume":"152","author":"Vaalamo","year":"1998","journal-title":"Am J Pathol"},{"issue":"21","key":"2024093006063257100_bib13","first-page":"491","article-title":"Matrix metalloprotineases","volume":"274","author":"Nagase","year":"1999","journal-title":"J Biol Chem"},{"key":"2024093006063257100_bib14","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1046\/j.1365-2168.1998.00556.x","article-title":"Matrix metalloproteinase 9 level predicts optimal collagen deposition during early wound repair in humans (erratum in Br J Surg 1998; 85: 715)","volume":"85","author":"\u00c5gren","year":"1998","journal-title":"Br J Surg"},{"key":"2024093006063257100_bib15","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1006\/jsre.1998.5495","article-title":"MMP-8 is the predominant collagenase in healing wounds and nonhealing ulcers","volume":"81","author":"Nwomeh","year":"1999","journal-title":"J Surg Res"},{"issue":"23","key":"2024093006063257100_bib16","first-page":"959","article-title":"Collagenase 2 (MMP-8) expression in murine tissue-remodeling processes. Analysis of its potential role in postpartum involution of the uterus","volume":"273","author":"Balbin","year":"1998","journal-title":"J Biol Chem"},{"key":"2024093006063257100_bib17","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/S0039-6060(98)70125-4","article-title":"Activation of tumor cell matrix metalloproteinase-2 by neutrophil proteinases requires expression of membrane-type 1 matrix metalloproteinase","volume":"124","author":"Schwartz","year":"1998","journal-title":"Surgery"},{"key":"2024093006063257100_bib18","first-page":"716","article-title":"Neutropenia prevents decrease in strength of rat intestinal anastomosis: partial effect of oxygen free radical scavengers and allopurinol","volume":"99","author":"H\u00f6gstr\u00f6m","year":"1986","journal-title":"Surgery"},{"key":"2024093006063257100_bib19","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1007\/BF01851851","article-title":"Early decrease in suture line breaking strength. The effect of proposed collagenase inhibition","volume":"185","author":"H\u00f6gstr\u00f6m","year":"1985","journal-title":"Res Exp Med"},{"key":"2024093006063257100_bib20","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1111\/j.1749-6632.1999.tb07697.x","article-title":"Matrix metalloproteinase inhibition. From the Jurassic to the third millennium","volume":"878","author":"Woessner","year":"1999","journal-title":"Ann N Y Acad Sci"},{"key":"2024093006063257100_bib21","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s000110050175","article-title":"Anti-arthritic activity of hydroxamic acid-based pseudopeptide inhibitors of matrix metalloproteinases and TNF alpha processing","volume":"46","author":"DiMartino","year":"1997","journal-title":"Inflamm Res"},{"key":"2024093006063257100_bib22","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/0014-5793(92)80300-6","article-title":"A novel coumarin-labelled peptide for sensitive continuous assays of the matrix metalloproteinases","volume":"296","author":"Knight","year":"1992","journal-title":"FEBS Lett"},{"key":"2024093006063257100_bib23","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/1523-1747.ep12506462","article-title":"Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker","volume":"78","author":"Bradley","year":"1982","journal-title":"J Invest Dermatol"},{"key":"2024093006063257100_bib24","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/0009-8981(80)90192-8","article-title":"Modified assay for determination of hydroxyproline in a tissue hydrolyzate","volume":"104","author":"Edwards","year":"1980","journal-title":"Clin Chim Acta"},{"key":"2024093006063257100_bib25","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/0014-4800(85)90090-5","article-title":"Loss of collagen from experimental intestinal anastomoses: early events","volume":"42","author":"Hendriks","year":"1985","journal-title":"Exp Mol Pathol"},{"key":"2024093006063257100_bib26","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1006\/exmp.1995.1017","article-title":"Intestinal anastomosis healing in rat: collagen concentrations and histochemical characterization by Picrosirius red staining and polarizing microscopy","volume":"62","author":"Rabau","year":"1995","journal-title":"Exp Mol Pathol"},{"key":"2024093006063257100_bib27","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1007\/BF02048337","article-title":"Collagen structural organization of healing colonic anastomoses and the effect of growth hormone treatment","volume":"38","author":"Christensen","year":"1995","journal-title":"Dis Colon Rectum"},{"key":"2024093006063257100_bib28","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/S0039-6060(98)70154-0","article-title":"Metalloproteinase inhibitors and wound healing: a novel enhancer of wound strength","volume":"124","author":"Witte","year":"1998","journal-title":"Surgery"},{"key":"2024093006063257100_bib29","doi-asserted-by":"crossref","first-page":"615","DOI":"10.1083\/jcb.148.3.615","article-title":"Role of cell surface metalloprotease MT1-MMP in epithelial cell migration over laminin-5","volume":"148","author":"Koshikawa","year":"2000","journal-title":"J Cell Biol"},{"key":"2024093006063257100_bib30","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s004030050459","article-title":"Matrix metalloproteinases (MMPs) are required for re-epithelialization of cutaneous wounds","volume":"291","author":"\u00c5gren","year":"1999","journal-title":"Arch Dermatol Res"},{"key":"2024093006063257100_bib31","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1681\/ASN.V93397","article-title":"Inhibition of matrix metalloproteinases attenuates anti-Thy1.1 nephritis","volume":"9","author":"Steinmann-Niggli","year":"1998","journal-title":"J Am Soc Nephrol"}],"container-title":["Journal of British Surgery"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2168.2001.01649.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2168.2001.01649.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/bjs\/article-pdf\/88\/2\/228\/59441152\/bjs398.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/bjs\/article-pdf\/88\/2\/228\/59441152\/bjs398.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,30]],"date-time":"2024-09-30T06:10:57Z","timestamp":1727676657000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/bjs\/article\/88\/2\/228\/6268130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,2,1]]},"references-count":31,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2001,2,1]]},"published-print":{"date-parts":[[2001,2,1]]}},"URL":"https:\/\/doi.org\/10.1046\/j.1365-2168.2001.01649.x","relation":{},"ISSN":["0007-1323","1365-2168"],"issn-type":[{"value":"0007-1323","type":"print"},{"value":"1365-2168","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2001,2]]},"published":{"date-parts":[[2001,2,1]]}}}