{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,28]],"date-time":"2025-07-28T21:44:09Z","timestamp":1753739049790},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":692,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p>The progression of cells from G<jats:sub>2<\/jats:sub> into mitosis is mainly controlled by formation of the cyclin B1\/p34<jats:sup>cdc2<\/jats:sup> complex. The behaviour of this complex in the irradiation\u2010induced G<jats:sub>2<\/jats:sub> cell cycle delay is still unclear. A prior study demonstrated that the expression of the cyclin B1 protein is reduced by irradiation, and restored to control levels by the methylxanthine drug pentoxifylline, which is a potent G<jats:sub>2<\/jats:sub> block abrogator. The present study shows that irradiation, and 2\u2003mM pentoxifylline affect the expression of the cyclin\u2010dependent kinase p34<jats:sup>cdc2<\/jats:sup> in HeLa cells. Irradiation induces p34<jats:sup>cdc2<\/jats:sup> levels to increase and cyclin B1 levels to decrease. Addition of pentoxifylline at the G<jats:sub>2<\/jats:sub> maximum reverses these trends. This is also evident from the cyclin B1\/p34<jats:sup>cdc2<\/jats:sup> ratios which decline after irradiation and are rapidly restored to control levels upon addition of pentoxifylline. It is concluded that cyclin B1 and p34<jats:sup>cdc2<\/jats:sup> protein expression are important events and act in concert to control the irradiation induced G<jats:sub>2<\/jats:sub> block. Analysis of cyclin B1 expression in whole cells and in isolated nuclei furthermore show that cyclin B1 is translocated from the nucleus into the cytoplasm when the G<jats:sub>2<\/jats:sub> block is abrogated by pentoxifylline.<\/jats:p>","DOI":"10.1046\/j.1365-2184.2000.00160.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T13:38:25Z","timestamp":1047389905000},"page":"39-50","source":"Crossref","is-referenced-by-count":13,"title":["Influence of the G<sub>2<\/sub> cell cycle block abrogator pentoxifylline on the expression and subcellular location of cyclin B1 and p34<sup>cdc2<\/sup> in HeLa cervical carcinoma cells"],"prefix":"10.1111","volume":"33","author":[{"given":"T.","family":"Theron","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. 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