{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T03:56:00Z","timestamp":1785902160926,"version":"3.56.0"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":723,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p> <jats:italic>Pityrosporum<\/jats:italic>\n          <jats:italic>orbiculare<\/jats:italic>, although a part of our normal cutaneous microflora, can cause skin infections and induce specific immunoglobulin (Ig) E antibodies in atopic dermatitis patients. <jats:italic>P. orbiculare<\/jats:italic> is therefore considered to be one of the trigger factors for atopic dermatitis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate if <jats:italic>P. orbiculare<\/jats:italic> can induce an eczematous reaction in atopic dermatitis patients, seborrhoeic dermatitis patients and healthy controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifteen atopic dermatitis patients, eight seborrhoeic dermatitis patients and eight healthy controls were patch tested with extract of <jats:italic>P. orbiculare<\/jats:italic> on non\u2010lesional, tape\u2010stripped skin of the back. NaCl was used as a negative control. The patch tests were evaluated after 24, 48 and 72\u2003h. Skin biopsies were taken from <jats:italic>P. orbiculare<\/jats:italic> patch test sites at 24\u2003h and 72\u2003h, from NaCl patch test sites at 72\u2003h, from non\u2010lesional skin and, in the atopic dermatitis patients, also from lesional skin. The skin biopsies were investigated with immunohistochemical techniques. <jats:italic>P. orbiculare<\/jats:italic>\u2010specific IgE in serum was analysed with RAST.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Specific IgE to <jats:italic>P. orbiculare<\/jats:italic> was found in serum from 13\/15 atopic dermatitis patients and in eight of them a positive patch test reaction to <jats:italic>P. orbiculare<\/jats:italic> was observed, with a maximal reaction at 48\u2003h. Significantly higher serum levels of <jats:italic>P. orbiculare<\/jats:italic>\u2010specific IgE were detected in patch test\u2010positive compared with patch test\u2010negative atopic dermatitis patients (<jats:italic>P<\/jats:italic> &lt;\u20030.01). The seborrhoeic dermatitis patients and healthy controls were RAST and patch test\u2010negative for <jats:italic>P. orbiculare<\/jats:italic>. In the patch test\u2010positive atopic dermatitis patients an infiltration of CD4<jats:sup>+<\/jats:sup>\u2003T cells and eosinophils was observed at the <jats:italic>P. orbiculare<\/jats:italic> patch test sites together with an upregulation of ICAM\u20101 and HLA\u2010DR expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p> <jats:italic>P. orbiculare<\/jats:italic> can induce an eczematous reaction in sensitized atopic dermatitis patients and may be an important trigger factor in these patients. The <jats:italic>P. orbiculare<\/jats:italic> patch test can be of diagnostic value in this subgroup of atopic dermatitis patients.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2222.2000.00702.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T09:40:29Z","timestamp":1047375629000},"page":"122-131","source":"Crossref","is-referenced-by-count":69,"title":["Positive atopy patch test reactions to <i>Pityrosporum<\/i><i>orbiculare<\/i> in atopic dermatitis patients"],"prefix":"10.1111","volume":"30","author":[{"family":"Tengvall 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