{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T21:54:08Z","timestamp":1773870848725,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":632,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p> <jats:italic>Anisakis simplex<\/jats:italic> is a seafood\u2010borne parasite that may both infect humans and cause allergy. Serodiagnosis of anisakiasis and allergy caused by this nematode is difficult since most <jats:italic>Anisakis<\/jats:italic> antigens show cross\u2010reactivity problems.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To analyse the possible role of sugar epitopes contained in <jats:italic>Anisakis simplex <\/jats:italic>antigens as causes of false\u2010positive results in serodiagnostic assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The antigens UA2R and UA3R recognized by two anti\u2010<jats:italic>Anisakis<\/jats:italic> monoclonal antibodies were used in this study. Capture ELISA techniques were used to compare the reactivities with native or <jats:italic>O<\/jats:italic>\u2010deglycosylated antigens of sera from <jats:italic>Anisakis<\/jats:italic>\u2010free children (most of them infected by several other parasites) and from <jats:italic>Anisakis<\/jats:italic> allergy patients. <jats:italic>O\u2010<\/jats:italic>deglycosylation was done by mild alkali treatment with NaOH. SDS\u2010PAGE and immunoblotting were used to characterize the effects of NaOH or <jats:italic>N<\/jats:italic>\u2010glycanase F treatment on UA3R.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Native UA2R was recognized by IgG1 and IgM antibodies in the sera of both <jats:italic>Anisakis<\/jats:italic>\u2010free subjects and allergy patients. Native UA3R was recognized by most sera from allergy patients (92% considering immunoglobulin (Ig) G1, 100% considering IgE), but also by a significant proportion of sera from <jats:italic>Anisakis<\/jats:italic>\u2010free subjects (36% considering IgG1, 14% considering IgE). <jats:italic>O<\/jats:italic>\u2010deglycosylation of UA3R greatly improved specificity: none of the sera from <jats:italic>Anisakis<\/jats:italic>\u2010free patients showed either IgG1 or IgE reactivity with <jats:italic>O<\/jats:italic>\u2010deglycosylated UA3R, while the proportion of sera from allergy patients showing IgE reactivity with this antigen was practically unaffected. <jats:italic>O<\/jats:italic>\u2010deglycosylation of UA2R did not improve the specificity of assays using this antigen. Our results also show that the protein core of glycoproteins may be altered by even very mild alkali treatment, depending on the nature of the protein.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Native glycoproteins of<jats:italic> A. simplex <\/jats:italic>should not be used for diagnostic purposes. <jats:italic>O<\/jats:italic>\u2010deglycosylated UA3R seems to be an excellent candidate for use as target antigen in the serodiagnosis of anisakiasis and <jats:italic>A. simplex<\/jats:italic> allergy.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2222.2000.00758.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T09:42:35Z","timestamp":1047375755000},"page":"551-559","source":"Crossref","is-referenced-by-count":37,"title":["<i>O<\/i>\u2010glycans as a source of cross\u2010reactivity in determinations of human serum antibodies to <i>Anisakis simplex<\/i> antigens"],"prefix":"10.1111","volume":"30","author":[{"family":"Lorenzo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Romar\u00eds","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Iglesias","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Aud\u00edcana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Alonso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Leiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Ubeira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"IshikuraH KikuchiK NagasawaKAnisakidae and anisakidosis. 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