{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:11:33Z","timestamp":1765545093769},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,9,23]],"date-time":"2008-09-23T00:00:00Z","timestamp":1222128000000},"content-version":"vor","delay-in-days":3067,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Activated eosinophils play an important role in the pathogenesis of bronchial asthma and other allergic diseases, and platelet\u2010activating factor (PAF) is a potent activator of eosinophils.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To characterize the cytosolic Ca<jats:sup>2+<\/jats:sup>\u2003([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>) mobilization in human eosinophils in response to PAF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>[Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> responses to PAF were examined in human eosinophils using a microscopic fura\u20102 fluorescence\u2010ratio imaging system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PAF caused a significant and dose\u2010dependent increase in (Ca<jats:sup>2+<\/jats:sup>)<jats:sub>i<\/jats:sub>, which consisted of an initial rapid rise followed by a sustained elevation. This PAF\u2010induced (Ca<jats:sup>2+<\/jats:sup>)<jats:sub>i<\/jats:sub> rise was inhibited by WEB 2086, a specific PAF receptor antagonist. The addition of 5\u2003m\n\t\t\t\t\t<jats:sc>m<\/jats:sc> EGTA or 1\u2003m\n\t\t\t\t\t<jats:sc>m<\/jats:sc> Ni<jats:sup>2+<\/jats:sup>\u2003to a nominally Ca<jats:sup>2+<\/jats:sup>\u2010free solution did not appreciably reduce the initial rise but significantly inhibited the sustained rise. The application of a protein kinase C inhibitor, Ro31\u20108220, augmented the sustained increase by PAF. Thapsigargin, a microsomal Ca<jats:sup>2+<\/jats:sup> ATPase inhibitor, induced no appreciable change in a nominally Ca<jats:sup>2+<\/jats:sup>\u2010free solution but induced a marked increase in (Ca<jats:sup>2+<\/jats:sup>)<jats:sub>i<\/jats:sub> when changed to a Ca<jats:sup>2+<\/jats:sup>\u2010containing solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The initial rapid rise and the following sustained rise in (Ca<jats:sup>2+<\/jats:sup>)<jats:sub>i<\/jats:sub> by PAF depends on Ca<jats:sup>2+<\/jats:sup> release from the intracellular Ca<jats:sup>2+<\/jats:sup> stores and Ca<jats:sup>2+<\/jats:sup> influx, respectively, which are regulated by protein kinase C in human eosinophils. Furthermore, the so called Ca<jats:sup>2+<\/jats:sup>\u2010capacitative entry is possibly involved in the Ca<jats:sup>2+<\/jats:sup> influx from the extracellular solution in human eosinophils.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2222.2000.00786.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T09:43:28Z","timestamp":1047375808000},"page":"699-705","source":"Crossref","is-referenced-by-count":16,"title":["Characterization of platelet\u2010activating factor\u2010induced cytosolic calcium mobilization in human 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