{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:34:56Z","timestamp":1761057296214},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":602,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The dust mites <jats:italic>Lepidoglyphus destructor<\/jats:italic> and <jats:italic>Tyrophagus putrescentiae<\/jats:italic> are important sources of allergen in farming environments. The major allergens of the dust mites <jats:italic>L<\/jats:italic>. <jats:italic>destructor<\/jats:italic> and <jats:italic>T. putrescentiae <\/jats:italic>have been cloned and expressed as recombinant proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the use of recombinant group 2 allergens of <jats:italic>L. destructor<\/jats:italic> (rLep d 2) and <jats:italic>T. putrescentiae<\/jats:italic> (rTyr p 2) in skin prick test (SPT), and serological analysis in sensitized and non\u2010sensitized farmers chronically exposed to dust mites.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Skin prick test with rLep d 2, rTyr p 2 and the corresponding commercial extracts was performed in 44 farmers sensitized to <jats:italic>L. destructor<\/jats:italic> and\/or <jats:italic>T. putrescentiae<\/jats:italic>, and 38 control farmers. IgE and IgG subclass antibodies to the recombinant allergens were analysed by RAST and ELISA, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of the 44 subjects positive in SPT to <jats:italic>L. destructor<\/jats:italic> and\/or <jats:italic>T. putrescentiae<\/jats:italic> extract, 26 (59%) displayed a positive SPT to one or the other of the recombinant allergens, whereas 21 (48%) were positive to both. Significant correlations were registered between the sizes of the weals induced by rLep d 2 and rTyr p 2 and the corresponding RAST values (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). A majority of subjects positive in SPT to the recombinant allergens had detectable IgG4 antibodies, and the levels were significantly higher in the dust mite sensitized group than in the controls (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.05). No such differences were found in the IgG1values (<jats:italic>P<\/jats:italic>\u2003&gt;\u20030.05). The results obtained with rLep d 2 and rTyr p 2 correlated relatively well with each other with respect to SPT, RAST and IgG4, suggesting that the allergens have similar or shared IgE epitopes. All the control subjects had a negative SPT and RAST to rLep d 2 and rTyr p 2.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Recombinant group 2 allergens from the dust mite <jats:italic>L. destructor<\/jats:italic> and <jats:italic>T. putrescentiae<\/jats:italic> represent useful tools for diagnosis of dust mite allergy.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2222.2000.00822.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T09:43:28Z","timestamp":1047375808000},"page":"670-676","source":"Crossref","is-referenced-by-count":18,"title":["Skin prick test and serological analysis with recombinant group 2 allergens of the dust mites <i>L. destructor<\/i> and <i>T. putrescentiae<\/i>"],"prefix":"10.1111","volume":"30","author":[{"family":"Kronqvist","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Johansson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Magnusson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Olsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Eriksson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Gafvelin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Van Hage\u2010Hamsten","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1997.d01-416.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1995.tb01167.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1987.tb02035.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00452.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1994.tb00245.x"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb03917.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1159\/000234468"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1994.tb00985.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)63060-8"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(95)98164-E"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1998.00375.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2510443.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","unstructured":"JohanssonE ErikssonTLJ OlssonSEvaluation of specific IgE to the recombinant group 2 mite allergens Lep d 2 and Tyr p 2 in the Pharmacia CAP System.Int Arch Allergy Immunol 1999; in press:.","DOI":"10.1159\/000024218"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000024026"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1159\/000231002"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(95)70102-8"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1159\/000237307"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/01647959708683109"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1996.tb00055.x"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1989.tb02431.x"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1995.tb01181.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70077-1"},{"key":"e_1_2_6_24_2","first-page":"63","article-title":"Analysis of house dust mite\u2010specific IgE, IgG4, and IgG antibodies during immunotherapy in asthmatic children","volume":"67","author":"Lin KL","year":"1991","journal-title":"Ann Allergy"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)70309-3"},{"key":"e_1_2_6_26_2","doi-asserted-by":"crossref","first-page":"722","DOI":"10.4049\/jimmunol.130.2.722","article-title":"Serological aspects of IgG4 antibodies. 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