{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:34:57Z","timestamp":1761057297371},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2002,4,5]],"date-time":"2002-04-05T00:00:00Z","timestamp":1017964800000},"content-version":"vor","delay-in-days":551,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Latex allergy can cause serious, preventable work\u2010related health problems in healthcare workers who are a high risk group for this form of allergy. Type I hypersensitivity can produce life\u2010threatening systemic effects, and involves an allergen\u2010specific immunoglobulin (IgE) response to proteins found in latex. The estimated prevalence of latex \u2018allergy\u2019 in healthcare workers varies widely (2.8% \u2212 18%), and studies do not always distinguish between those who are positive in an assay for latex\u2010specific IgE and those with clinical allergy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess the performance of four <jats:italic>in\u2010vitro<\/jats:italic> methods and three skin testing methods for detecting latex\u2010specific IgE in a group of UK healthcare workers. Test results were compared with reported clinical symptoms defined by questionnaire.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Skin prick testing was carried out on volunteers using three reagents: (a) stallergenes commercial latex extract (Cedex, France); (b) an in\u2010house latex glove extract; and (c) a fresh glove piece. Specific IgE levels were determined using Pharmacia Autocap<jats:sup>TM<\/jats:sup> (Uppsala, Sweden), Pharmacia Unicap<jats:sup>TM<\/jats:sup> (Uppsala, Sweden), DPC Immulite<jats:sup>\u00ae<\/jats:sup> (Los Angeles, USA) and Hycor Hytec<jats:sup>TM<\/jats:sup> (Irvine, California, USA) methods. Each volunteer completed a questionnaire detailing latex exposure and allergic history.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p> <jats:italic>In vitro<\/jats:italic> methods for detecting specific IgE to natural rubber latex were positive in 3.6%, to 43.6% of the same population. Skin prick tests positivity varied between 2.9% and 14.3% with different extracts. From the subjects tested 9.1% reported symptoms which could be consistent with type I allergy, although none had been given a pre\u2010existing diagnosis of latex allergy, and 43.6% of volunteers reported symptoms consistent with type IV hypersensitivity or irritant dermatitis. Contingency tables and chi\u2010squared analysis revealed no correlation between most methods. No correlation was shown between symptoms consistent with type I allergy and any <jats:italic>in vitro<\/jats:italic> or skin testing method for latex\u2010specific IgE.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A wide variation between testing procedures was found, and no method could be correlated with reported symptoms of type I allergy. At least one <jats:italic>in vitro<\/jats:italic> specific IgE assay produced a high percentage of positive results at variance with the clinical symptoms in volunteers. A clinical history is essential in establishing type I hypersensitivity to latex and test results should not be used in isolation. The incidence of clinical sensitization may be seriously over\u2010estimated if only laboratory parameters are used.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2222.2000.00908.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T09:41:05Z","timestamp":1047375665000},"page":"1444-1449","source":"Crossref","is-referenced-by-count":20,"title":["Assessment of latex allergy in a healthcare population: are the available tests valid?"],"prefix":"10.1111","volume":"30","author":[{"given":"C.","family":"Pridgeon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Wild","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Ashworth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Egner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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