{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T01:47:40Z","timestamp":1789350460158,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,5,30]],"date-time":"2002-05-30T00:00:00Z","timestamp":1022716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background<\/jats:bold> Interleukin\u20109 is a T cell\u2010derived Th2\u2010type cytokine that has been linked to airway hyper\u2010responsiveness, mucus hypersecretion and mast cell infiltration in animal models. We recently demonstrated the potential for IL\u20109 to act in human eosinophil development and survival.<\/jats:p><jats:p><jats:bold>Objectives<\/jats:bold> The aims of this study were: (i) to compare IL\u20109 mRNA expression in bronchial biopsies between atopic asthmatics and normal controls, (ii) to investigate kinetic expression of IL\u20109 mRNA in skin biopsies after allergen challenge; and (iii) to relate IL\u20109 expression to infiltration of eosinophils, mast cell and T lymphocytes in local tissue.<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold> Bronchial biopsies were obtained from atopic asthmatics (<jats:italic>n<\/jats:italic>\u2003=\u200312) and normal non\u2010asthmatics (<jats:italic>n<\/jats:italic>\u2003=\u200312) at baseline. Skin biopsies were obtained from atopic subjects (<jats:italic>n<\/jats:italic>\u2003=\u200311) at 1, 3, 6, 24, 48 and 72\u2003h after allergen challenge. Diluent challenge sites at 24\u2003h were used as controls. IL\u20109 mRNA was identified using the technique of <jats:italic>in situ<\/jats:italic> hybridization. The numbers of eosinophils, mast cells and T cells were evaluated by immunohistochemistry.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold> The numbers of IL\u20109 mRNA<jats:sup>+<\/jats:sup> cells present in the bronchial mucosa were significantly greater in atopic asthmatics than those in normal controls (<jats:italic>P<\/jats:italic>\u2003=\u20030.003). The numbers of eosinophils, but not mast cells, were also significantly higher in asthmatics (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.005). The numbers of IL\u20109 mRNA<jats:sup>+<\/jats:sup> cells present in the airway of asthmatics significantly correlated with the numbers of eosinophils (<jats:italic>r<\/jats:italic>\u2003=\u20030.623, <jats:italic>P<\/jats:italic>\u2003=\u20030.03), but not mast cells or T cells. Compared with diluent challenge, the numbers of IL\u20109 mRNA<jats:sup>+<\/jats:sup> cells were significantly elevated at all allergen\u2010challenged sites in the skin, with maximal signals at 48\u2003h (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.005). At 72\u2003h, the numbers of IL\u20109 mRNA<jats:sup>+<\/jats:sup> cells significantly correlated with the numbers of eosinophils (<jats:italic>r<\/jats:italic>\u2003=\u20030.707, <jats:italic>P<\/jats:italic>\u2003=\u20030.015).<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold> Elevated expression of IL\u20109 in allergic inflammation may contribute to local eosinophil infiltration and survival in asthma and other allergic atopic diseases.<\/jats:p>","DOI":"10.1046\/j.1365-2222.2002.01376.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T09:51:39Z","timestamp":1047376299000},"page":"866-871","source":"Crossref","is-referenced-by-count":42,"title":["Elevated expression of interleukin\u20109 mRNA in the bronchial mucosa of atopic asthmatics and allergen\u2010induced cutaneous late\u2010phase reaction: relationships to eosinophils, mast cells and T lymphocytes"],"prefix":"10.1111","volume":"32","author":[{"given":"S.","family":"Ying","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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