{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:32:34Z","timestamp":1791405154524,"version":"4.3.3"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[1999,8,1]],"date-time":"1999-08-01T00:00:00Z","timestamp":933465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,24]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Lymphocyte transformation test has often been used as an in\u2003vitro test for nickel allergy. We have previously demonstrated the presence of nickel-reactive T cells in individuals with no history of allergic disease and with a negative patch test towards NiSO4. In this study, we show that this proliferative response was mainly confined to T cells within the CD4+ subset. In contrast to conventional recall antigens such as tetanus toxoid, in vitro stimulation using NiSO4 activated both FACS-purified CD4+CD45RA+\u2018naive\u2019 and CD4+CD45RO+\u2018memory\u2019 T cells. To determine which cell population reacted with nickel to induce T cell activation, peripheral blood mononuclear cells were separated into macrophages and non-adherent, HLA-DR-depleted T cells. We found that preincubation of monocytes\/macrophages but not T cells with NiSO4 resulted in subsequent T cell proliferation. This result demonstrated that nickel did not exhibit any direct effect on the T cell. Furthermore, the NiSO4-induced T cell proliferation could be blocked by antibodies towards MHC class II (HLA-DR) molecules. Our results substantiate the concept that individuals with a negative patch test towards NiSO4 contain in their peripheral blood T cells capable of recognizing nickel or nickel-modified peptides. In contrast to conventional recall antigens, both memory and naive T cells were activated. Thus, when compared with data obtained from nickel-allergic individuals, this study shows a comparable nickel-inducible T cell activation in non-allergic individuals.<\/jats:p>","DOI":"10.1046\/j.1365-2249.1999.00967.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:16:45Z","timestamp":1047377805000},"page":"217-222","source":"Crossref","is-referenced-by-count":29,"title":["Nickel-induced proliferation of both memory and naive T cells in patch test-negative individuals"],"prefix":"10.1093","volume":"117","author":[{"given":"S","family":"Lisby","sequence":"first","affiliation":[{"name":"Department of Dermatology, University of Copenhagen, Gentofte Hospital, Hellerup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L H","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of Copenhagen, Gentofte Hospital, Hellerup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Menn\u00e9","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of Copenhagen, Gentofte Hospital, Hellerup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O","family":"Baadsgaard","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of Copenhagen, Gentofte Hospital, Hellerup, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"2022011519162891900_b1","doi-asserted-by":"crossref","first-page":"456","DOI":"10.2340\/0001555572456460","article-title":"Allergic contact sensitization in an unselected Danish population","volume":"72","author":"Nielsen","year":"1992","journal-title":"Acta Derm Venereol 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