{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:37:55Z","timestamp":1771475875427,"version":"3.50.1"},"reference-count":22,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,4,5]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>A subset of pemphigus herpetiformis, a rare pemphigus variant, is characterized histopathologically by subcorneal acantholysis and neutrophilic infiltration. The mechanism of neutrophil infiltration is unknown, but chemokines such as IL-8 may play a role. We investigated the possible role of IL-8 in two such cases. Direct and indirect immunofluorescence studies demonstrated in vivo-bound and circulating IgG epithelial cell surface-binding autoantibodies, both predominated by IgG4 subclass. ELISA and immunoblotting studies revealed that the patients\u2019 IgG autoantibodies recognized recombinant desmoglein 1 but not desmoglein 3. Preadsorption of the patients\u2019 sera with recombinant desmoglein 1 completely removed the epidermal cell surface immunostaining. Significantly, immunohistochemistry demonstrated intense expression of IL-8, co-localized with in vivo-bound IgG, in the upper epidermis, where the acantholysis took place. Affinity-purified sera IgG from these two patients, a normal individual, and a pemphigus vulgaris patient containing desmoglein 1 autoantibodies, were incubated with normal human keratinocytes in vitro. Cells treated with these patients\u2019 IgG secreted a seven-to-nine-fold increase of IL-8 (30\u201337\u2003pg\/ml) compared with the controls (2\u20134\u2003pg\/ml) and expressed a higher intensity of cytoplasmic IL-8 staining. These data demonstrate a novel functional role for IL-8 in the pathogenesis of the neutrophil-dominant subset of pemphigus herpetiformis. The autoantibody-induced epidermal cell IL-8 expression may represent a novel mechanism of epidermal neutrophil recruitment.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2000.01104.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:19:33Z","timestamp":1047377973000},"page":"217-224","source":"Crossref","is-referenced-by-count":48,"title":["Induction of keratinocyte IL-8 expression and secretion by IgG autoantibodies as a novel mechanism of epidermal neutrophil recruitment in a pemphigus variant"],"prefix":"10.1093","volume":"119","author":[{"given":"E A","family":"O\u2019Toole","sequence":"first","affiliation":[{"name":"Departments of Dermatology, North-western University Medical School, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L L","family":"Mak","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, North-western University Medical School, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Guitart","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, North-western University Medical School, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D T","family":"Woodley","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, North-western University Medical School, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Kurume University School of Medicine, Kurume"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Amagai","sequence":"additional","affiliation":[{"name":"Keio University Medical School, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L S","family":"Chan","sequence":"additional","affiliation":[{"name":"Departments of Dermatology, North-western University Medical School, Chicago, IL, USA"},{"name":"Medicine Service, Section of Dermatology, Lakeside Division, VA Chicago Health Care System, Chicago, IL, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2002,4,5]]},"reference":[{"key":"b1_1145","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90360-B"},{"key":"b2_1146","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12606168"},{"key":"b3_1147","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1172\/JCI5252","volume":"103","author":"Mahoney MG","year":"1999","journal-title":"J Clin Invest"},{"key":"b4_1148","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1111\/j.1365-4362.1975.tb00125.x","volume":"14","author":"Jablonska S","year":"1975","journal-title":"Int J Dermatol"},{"key":"b5_1149","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1111\/j.1600-0560.1996.tb01477.x","volume":"23","author":"Huhn KM","year":"1996","journal-title":"J Cutan Pathol"},{"key":"b6_1150","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1016\/S0190-9622(96)90832-4","volume":"34","author":"Santi CG","year":"1996","journal-title":"J Am Acad Dermatol"},{"key":"b7_1151","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1997.17781865.x"},{"key":"b8_1152","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12319025"},{"key":"b9_1153","first-page":"1901","volume":"150","author":"Wang J","year":"1997","journal-title":"Am J Pathol"},{"key":"b10_1154","doi-asserted-by":"crossref","first-page":"1760","DOI":"10.4049\/jimmunol.135.3.1760","volume":"135","author":"Hall RP","year":"1985","journal-title":"J Immunol"},{"key":"b11_1155","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1111\/j.1365-2133.1994.tb08491.x","volume":"131","author":"Wojnarowska F","year":"1994","journal-title":"Br J Dermatol"},{"key":"b12_1156","doi-asserted-by":"publisher","DOI":"10.1038\/332647a0"},{"key":"b13_1157","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/BF01534385","volume":"19","author":"Zhang Y","year":"1995","journal-title":"Inflammation"},{"issue":"1","key":"b14_1158","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/jid.1995.5","volume":"105","author":"Schroder JM","year":"1995","journal-title":"J Invest Dermatol"},{"key":"b15_1159","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1997.3586"},{"key":"b16_1160","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1111\/1523-1747.ep12365189","volume":"101","author":"Chan LS","year":"1993","journal-title":"J Invest Dermatol"},{"key":"b17_1161","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","author":"Lowry OH","year":"1951","journal-title":"J Biol Chem"},{"key":"b18_1162","first-page":"129","volume":"138","author":"Nickoloff BJ","year":"1991","journal-title":"Am J Pathol"},{"key":"b19_1163","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.1997.d01-1180.x"},{"key":"b20_1164","doi-asserted-by":"crossref","first-page":"2010","DOI":"10.4049\/jimmunol.159.4.2010","volume":"159","author":"Ishii K","year":"1997","journal-title":"J Immunol"},{"key":"b21_1165","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12874456"},{"key":"b22_1166","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1038\/ng0695-161","volume":"10","author":"Hengge UR","year":"1995","journal-title":"Nature Genet"}],"container-title":["Clinical and Experimental Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2249.2000.01104.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2249.2000.01104.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/119\/1\/217\/42181659\/j.1365-2249.2000.01104.x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/119\/1\/217\/42181659\/j.1365-2249.2000.01104.x.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,22]],"date-time":"2023-04-22T03:03:38Z","timestamp":1682132618000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cei\/article\/119\/1\/217\/6461854"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,1]]},"references-count":22,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2002,4,5]]},"published-print":{"date-parts":[[2002,4,5]]}},"URL":"https:\/\/doi.org\/10.1046\/j.1365-2249.2000.01104.x","relation":{},"ISSN":["1365-2249","0009-9104"],"issn-type":[{"value":"1365-2249","type":"electronic"},{"value":"0009-9104","type":"print"}],"subject":[],"published-other":{"date-parts":[[2000,1]]},"published":{"date-parts":[[2000,1]]}}}