{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:36:17Z","timestamp":1791196577351,"version":"4.1.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,24]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Infectious mononucleosis (IM), a manifestation of primary infection with EBV, is characterized by a massive expansion of the T cell population. In this study we examined this expanded T cell population regarding its EBV status, its proliferative and apoptotic activity, and its expression of apoptosis-related genes. Whereas previous studies were performed on ex vivo cultures or on peripheral blood, our investigations included in vivo analysis of IM tonsillectomy specimens (14 cases) by in situ hybridization for viral RNA (EBERs) combined with immunohistochemistry (IHC; CD3, CD45RO, CD20, CD79a, Ki-67, Bcl-2, Bax, Fas, FasL) and the TUNEL method. Of the EBER+ cells 50\u201370% showed expression of the B cell markers CD20\/CD79a. The remainder of the EBER+ cells expressed neither B nor T cell antigens. No co-expression of EBERs and T cell antigens was detected in any of the specimens. In accordance with a high rate of apoptosis (up to 2.37%) within the expanded T cell population, Bcl-2 expression was drastically reduced and FasL expression remarkably increased. The levels of Bax and Fas expression showed no or moderate up-regulation. In conclusion, the massive expansion of IM T cells is not caused by EBV infection of these cells but merely represents an intense immune reaction. Through altered expression of Bcl-2\/Bax and Fas\/FasL, the activated T cells are subject to enhanced apoptosis while residing within the lymphoid tissue, which eventually allows the efficient silencing of this potentially damaging T cell response.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2000.01181.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:21:27Z","timestamp":1047378087000},"page":"294-300","source":"Crossref","is-referenced-by-count":23,"title":["Characterization of the expanded T cell population in infectious mononucleosis: apoptosis, expression of apoptosis-related genes, and Epstein\u2013Barr virus (EBV) status"],"prefix":"10.1093","volume":"120","author":[{"given":"C S","family":"Verbeke","sequence":"first","affiliation":[{"name":"Pathology Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U","family":"Wenthe","sequence":"additional","affiliation":[{"name":"Pathology Institute and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W F","family":"Bergler","sequence":"additional","affiliation":[{"name":"Department of Oto-Rhino-Laryngology, Faculty of Clinical Medicine, University of Heidelberg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Zentgraf","sequence":"additional","affiliation":[{"name":"Applied Tumourvirology, German Cancer Research Centre, Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"2022011221074611100_b1","first-page":"107","article-title":"Epstein-Barr virus","volume-title":"The human herpesviruses","author":"Liebowitz","year":"1993"},{"key":"2022011221074611100_b2","doi-asserted-by":"crossref","first-page":"8693","DOI":"10.1073\/pnas.85.22.8693","article-title":"Eradication of Epstein-Barr virus by allogeneic bone marrow transplantation: implications for sites of viral latency","volume":"85","author":"Gratama","year":"1988","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022011221074611100_b3","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/0046-8177(89)90075-0","article-title":"Identification of Epstein-Barr virus-infected cells in tonsils of acute infectious mononucleosis by in situ hybridization","volume":"20","author":"Niedobitek","year":"1989","journal-title":"Hum Pathol"},{"key":"2022011221074611100_b4","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1002\/(SICI)1096-9896(199706)182:2<151::AID-PATH824>3.0.CO;2-3","article-title":"Epstein-Barr virus (EBV) infection in infectious mononucleosis: virus latency, replication and phenotype of EBV-infected cells","volume":"182","author":"Niedobitek","year":"1997","journal-title":"J Pathol"},{"key":"2022011221074611100_b5","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1182\/blood.V85.3.744.bloodjournal853744","article-title":"Morphology, immunophenotype, and distribution of latently and\/or productively Epstein-Barr virus-infected cells in acute infectious mononucleosis: implications for the interindividual infection route of Epstein-Barr virus","volume":"85","author":"Anagnostopoulos","year":"1995","journal-title":"Blood"},{"key":"2022011221074611100_b6","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1056\/NEJM198803243181203","article-title":"T-cell lymphoma containing Epstein-Barr viral DNA in patients with chronic Epstein-Barr virus infection","volume":"318","author":"Jones","year":"1988","journal-title":"N Engl J Med"},{"key":"2022011221074611100_b7","doi-asserted-by":"crossref","first-page":"1444","DOI":"10.1172\/JCI116721","article-title":"Epstein-Barr virus-infected T lymphocytes in Epstein-Barr virus-associated hemophagocytic syndrome","volume":"92","author":"Kawaguchi","year":"1993","journal-title":"J Clin Invest"},{"key":"2022011221074611100_b8","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0065-230X(08)60319-X","article-title":"The association of Epstein-Barr virus (EBV) with T cell lymphoproliferations and Hodgkin\u2019s disease: two new developments in the EBV field","volume":"62","author":"Pallesen","year":"1994","journal-title":"Adv Cancer Res"},{"key":"2022011221074611100_b9","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1006\/scbi.1996.0027","article-title":"EBV infection of T cells: potential role in malignant transformation","volume":"7","author":"Kelleher","year":"1996","journal-title":"Sem Canc Biol"},{"key":"2022011221074611100_b10","first-page":"951","article-title":"Peripheral blood lymphocyte apoptosis. 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