{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:26:43Z","timestamp":1759332403564},"reference-count":25,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,24]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n               <jats:p>SP-A is found principally in the lung, and has been associated with lamellar bodies also found in the synovial joint. Both SP-A and C1q contain collagen-like regions, and SP-A and C1q have some structural similarities, both having a globular head region and a collagen-like tail. Here we are able to show that (i) autoreactivity to SP-A, as expressed by IgG and IgM autoantibodies, is present in synovial fluid (SF) isolated from patients with rheumatoid arthritis (RA); (ii) in absorption experiments only a limited degree of cross-reactivity between autoantibodies reactive with C1q and SP-A is observed; (iii) there is no cross-reactivity between autoantibodies reactive with type II collagen (CII) and those reactive with SP-A or C1q; (iv) autoantibodies react with polymeric (dimers and larger) SP-A, but not with monomeric SP-A subunits, indicating that a degree of quaternary structure is required for antibody binding. Unlike CII, which not accessible in the normal joint, both SP-A and C1q are available within the SF in patients with RA and may therefore provide antigens driving an autoimmune response directed against collagen-like structures.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2000.01185.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:20:51Z","timestamp":1047378051000},"page":"183-187","source":"Crossref","is-referenced-by-count":5,"title":["Humoral autoreactivity directed against surfactant protein-A (SP-A) in rheumatoid arthritis synovial fluids"],"prefix":"10.1093","volume":"120","author":[{"given":"P K E","family":"Trinder","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology & Hygiene, Johannes Gutenberg University, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T P","family":"Hickling","sequence":"additional","affiliation":[{"name":"MRC Immunochemistry Unit, Oxford University, Oxford, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R B","family":"Sim","sequence":"additional","affiliation":[{"name":"MRC Immunochemistry Unit, Oxford University, Oxford, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Brackertz","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, St Vincenz & Elizabeth Hospital, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Loos","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology & Hygiene, Johannes Gutenberg University, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M J","family":"Maeurer","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology & Hygiene, Johannes Gutenberg University, Mainz, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"2022011316464678200_b1","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0167-5699(94)90136-8","article-title":"Collectins: collagenous C-type lectins of the innate immune defense system","volume":"15","author":"Holmskov","year":"1994","journal-title":"Immunol Today"},{"key":"2022011316464678200_b2","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1002\/pro.5560030801","article-title":"Collectins\u2014soluble proteins containing collagenous regions and lectin domains\u2014their roles in innate immunity","volume":"3","author":"Hoppe","year":"1994","journal-title":"Protein Sci"},{"key":"2022011316464678200_b3","doi-asserted-by":"crossref","first-page":"467","DOI":"10.3109\/01902149609046036","article-title":"Characterization of radioiodinated lung surfactant protein A (SP-A) and the effects of oxidation on SP-A ultrastructure and activity","volume":"22","author":"Stuart","year":"1996","journal-title":"Exp Lung Res"},{"key":"2022011316464678200_b4","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1007\/BF03401923","article-title":"Human lung surfactant protein A (SP-A) exists in several oligomeric states: oligomer size distribution varies between patient groups","volume":"4","author":"Hickling","year":"1998","journal-title":"Mol Med"},{"key":"2022011316464678200_b5","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1177\/089686088900900314","article-title":"Mesothelium secretes lamellar bodies in a similar manner to Type II pneumocyte secretion of surfactant","volume":"9","author":"Dobbie","year":"1984","journal-title":"Perit Dial Int"},{"key":"2022011316464678200_b6","doi-asserted-by":"crossref","first-page":"508","DOI":"10.1093\/rheumatology\/33.6.508","article-title":"Lamellar bodies in synoviocytes, mesothelium and specific epithelia as possible site of autoantigen in rheumatoid disease","volume":"33","author":"Dobbie","year":"1994","journal-title":"Br J Rheumatol"},{"key":"2022011316464678200_b7","first-page":"574","article-title":"Surfactant protein A and lamellar bodies: a homologous secretory function of peritoneum, synovium and lung","volume":"16","author":"Dobbie","year":"1996","journal-title":"Perit Dial Int"},{"key":"2022011316464678200_b8","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1046\/j.1365-2567.1996.495559.x","article-title":"The collagen-like component of the complement system, C1q, is recognized by 7S autoantibodies and is functionally impaired in synovial fluids of patients with rheumatoid arthritis","volume":"87","author":"Trinder","year":"1996","journal-title":"Immunology"},{"key":"2022011316464678200_b9","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/0165-2478(93)90052-4","article-title":"Increased expression of C1q receptors on neutrophils from inflammatory joint fluids","volume":"36","author":"Crockard","year":"1993","journal-title":"Immunol Letters"},{"key":"2022011316464678200_b10","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J Biol Chem"},{"key":"2022011316464678200_b11","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/0022-1759(84)90361-2","article-title":"Purification and characterization of human, guinea pig and mouse C1q by fast protein liquid chromatography (FPLC)","volume":"74","author":"Stemmer","year":"1984","journal-title":"J Immunol Methods"},{"key":"2022011316464678200_b12","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1042\/bj2930873","article-title":"Studies on the carbohydrate-binding characteristics of human pulmonary surfactant-associated protein A and comparison with two other collectins: mannan binding protein and conglutinin","volume":"293","author":"Haurum","year":"1993","journal-title":"Biochem J"},{"key":"2022011316464678200_b13","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during the assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"2022011316464678200_b14","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1042\/bj3190329","article-title":"Improvements on the purification of mannose-binding lectin and demonstration of its Ca2+-independent association with a C1s-like serine protease","volume":"319","author":"Tan","year":"1996","journal-title":"Biochem J"},{"key":"2022011316464678200_b15","doi-asserted-by":"crossref","first-page":"1646","DOI":"10.1002\/art.1780331107","article-title":"IgG and IgA antibodies to the collagen-like region of C1q in rheumatoid vasculitis","volume":"33","author":"Siegert","year":"1990","journal-title":"Arthritis Rheum"},{"key":"2022011316464678200_b16","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1002\/art.1780310401","article-title":"Evidence for the presence of autoantibodies to the collagen-like portion of C1q in systemic lupus erythematosus","volume":"31","author":"Antes","year":"1988","journal-title":"Arthritis Rheum"},{"key":"2022011316464678200_b17","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1002\/anr.1780320506","article-title":"Antibodies to the collagen-like region of C1q in sera of patients with autoimmune rheumatic disease","volume":"32","author":"Wener","year":"1989","journal-title":"Arthritis Rheum"},{"key":"2022011316464678200_b18","doi-asserted-by":"crossref","first-page":"836","DOI":"10.1038\/ki.1989.61","article-title":"Antibody to a cryptic, solid phase C1q antigen in membranoproliferative nephritis","volume":"35","author":"Strife","year":"1989","journal-title":"Kidney Int"},{"key":"2022011316464678200_b19","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/anr.1780320910","article-title":"Serum IgG antibodies to C1q in hypocomplementemic urticarial vasculitis syndrome","volume":"32","author":"Wisnieski","year":"1989","journal-title":"Arthritis Rheum"},{"key":"2022011316464678200_b20","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/nm0395-237","article-title":"Glycosylation changes of IgG associated with rheumatoid arthritis can activate complement via the mannose-binding protein","volume":"1","author":"Malhotra","year":"1995","journal-title":"Nature Med"},{"key":"2022011316464678200_b21","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/0014-5793(88)80026-7","article-title":"Enzymatic alteration of C1q, the collagen-like subcomponent of the first component of complement, leads to cross-reactivity with type II collagen","volume":"228","author":"Heinz","year":"1988","journal-title":"FEBS Letters"},{"key":"2022011316464678200_b22","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/0161-5890(89)90098-9","article-title":"Common epitopes in C1q and collagen type II","volume":"26","author":"Heinz","year":"1989","journal-title":"Mol Immunol"},{"key":"2022011316464678200_b23","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1006\/jaut.1994.1026","article-title":"Antibodies to the collagen-like region of C1q and type II collagen are independent non-cross-reactive populations in systemic lupus erythematosus and rheumatoid arthritis","volume":"7","author":"Cook","year":"1994","journal-title":"J Autoimmun"},{"key":"2022011316464678200_b24","first-page":"109","article-title":"Chronicity of tissue-specific experimental autoimmune disease: a role for B cells?","volume-title":"Immunol Rev","author":"Holmdahl","year":"1995"},{"key":"2022011316464678200_b25","doi-asserted-by":"crossref","first-page":"314","DOI":"10.1046\/j.1365-3083.1996.d01-48.x","article-title":"Human autoantibodies against C1q: lack of cross-reactivity with the collectins mannan-binding protein, lung surfactant protein A and bovine conglutinin","volume":"43","author":"M\u00e5rtensson","year":"1996","journal-title":"Scand J Immunol"}],"container-title":["Clinical and Experimental Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2249.2000.01185.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2249.2000.01185.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/120\/1\/183\/42183077\/j.1365-2249.2000.01185.x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/120\/1\/183\/42183077\/j.1365-2249.2000.01185.x.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T11:47:21Z","timestamp":1676375241000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cei\/article\/120\/1\/183\/6461660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,4]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2001,12,24]]},"published-print":{"date-parts":[[2001,12,24]]}},"URL":"https:\/\/doi.org\/10.1046\/j.1365-2249.2000.01185.x","relation":{},"ISSN":["1365-2249","0009-9104"],"issn-type":[{"value":"1365-2249","type":"electronic"},{"value":"0009-9104","type":"print"}],"subject":[],"published-other":{"date-parts":[[2000,4]]},"published":{"date-parts":[[2000,4]]}}}