{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T16:41:01Z","timestamp":1787244061830,"version":"build-2736575974"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,24]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>Apoptosis in peripheral blood lymphocyte populations in HTLV-I-infected people in vivo was examined, to study the lymphocyte dynamics in HTLV-I infection. Freshly isolated lymphocytes from 10 non-infected healthy people, eight asymptomatic HTLV-I carriers and 15 patients with HAM\/TSP were stained with FITC-labelled annexin V to detect phosphatidylserine (PS) residue exposure at the outer plasma membrane leaflet as an early marker of apoptosis. There was no significant difference in annexin V positivity in CD4+ and CD8+ lymphocytes between non-infected subjects, asymptomatic carriers and HAM\/TSP patients, but there was a greatly increased exposure of PS on CD19+ lymphocytes (B cells) detected by FITC\u2013annexin V in 12 out of 15 (80%) HAM\/TSP patients, while only two out of eight (25%) asymptomatic carriers and none of the non-infected healthy people showed this aberrant PS exposure on B cells. The intensity of annexin V staining of B cells in HAM\/TSP was intermediate, as distinct from the high annexin V staining on advanced apoptotic cells. However, annexin V positivity was decreased when the cells were stained after 24\u2003h of culture, suggesting that the intermediate PS exposure on the B cell in HAM\/TSP is not a consequence of an apoptotic process, but rather reflects reversible membrane damage. B cells with PS exposure in vivo might provide a site for coagulation and inflammation, and so contribute to the pathogenesis of HAM\/TSP and its complications.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2000.01211.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T05:21:27Z","timestamp":1047360087000},"page":"307-316","source":"Crossref","is-referenced-by-count":16,"title":["Frequent reversible membrane damage in peripheral blood B cells in human T cell lymphotropic virus type I (HTLV-I)-associated myelopathy\/tropical spastic paraparesis (HAM\/TSP)"],"prefix":"10.1093","volume":"120","author":[{"given":"Y","family":"Furukawa","sequence":"first","affiliation":[{"name":"Department of Immunology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C R M","family":"Bangham","sequence":"additional","affiliation":[{"name":"Department of Immunology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G P","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of GU Medicine and Communicable Disease, Imperial College School of Medicine at St Mary\u2019s, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J N","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of GU Medicine and Communicable Disease, Imperial College School of Medicine at St Mary\u2019s, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Osame","sequence":"additional","affiliation":[{"name":"Third Department of Internal Medicine, Faculty of Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"2022011221075130000_b1","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0140-6736(85)92734-5","article-title":"Antibodies to human T-lymphotropic virus type-I in patients 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