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Expression of the 19-kD antigen as a recombinant protein in two saprophytic mycobacteria\u2014M.\u2003vaccae and M.\u2003smegmatis\u2014resulted in abrogation of their ability to confer protection against M.\u2003tuberculosis in a murine challenge model, and in their ability to prime a DTH response to cross-reactive mycobacterial antigens. Induction of an immune response to the 19-kD antigen by an alternative approach of DNA vaccination had no effect on subsequent M.\u2003tuberculosis challenge. These results are consistent with a model in which the presence of the 19-kD protein has a detrimental effect on the efficacy of vaccination with live mycobacteria. Targeted inactivation of genes encoding selected antigens represents a potential route towards development of improved vaccine candidates.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2000.01212.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:21:27Z","timestamp":1047378087000},"page":"274-279","source":"Crossref","is-referenced-by-count":43,"title":["The 19-kD antigen and protective immunity in a murine model of tuberculosis"],"prefix":"10.1093","volume":"120","author":[{"given":"V V","family":"Yeremeev","sequence":"first","affiliation":[{"name":"Laboratory for Immunogenetics, Central Institute for Tuberculosis, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I V","family":"Lyadova","sequence":"additional","affiliation":[{"name":"Laboratory for Immunogenetics, Central Institute for Tuberculosis, Moscow, Russia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B 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