{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T05:10:40Z","timestamp":1761196240313},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2000,8,1]],"date-time":"2000-08-01T00:00:00Z","timestamp":965088000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,10,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Chlamydia pneumoniae infection is associated with atherosclerosis and the organism has been identified in arterial lesions. To determine whether T lymphocyte-mediated immune responses to Chlamydia antigens within plaque could contribute to pathogenesis, we have derived T cell lines from atherosclerotic plaques of 32 patients. Culture with IL-2 alone proved insufficient for cellular activation and expansion, but additional stimulation with phytohaemagglutinin (PHA) or recall antigens allowed consistent establishment of T cell lines. Furthermore, in cultures of approx. 500 tissue fragments, Chlamydia organisms proved as effective as other recall antigens in producing outgrowth of arterial T cells (20\u201325% wells produced T cell lines). Testing the antigen responsiveness of T cell lines showed that those derived using Chlamydia organisms were more likely to respond to Chlamydia (5\/29+) than those isolated using other stimuli (6\/69+ for PHA; 5\/57+ for PPD and tetanus toxoid (TT)). However, lines responsive to each of the recall antigens were observed. Using recombinant Chlamydia antigens, some Chlamydia-specific T cell lines were shown to respond to OMP2 and\/or hsp60. Those recognizing Chlamydia hsp60 did not cross-react with human hsp60, but human hsp60-responsive lines were also observed. Thus, atherosclerotic plaque tissue contains a variety of memory T lymphocytes, and amongst these are cells capable of recognizing Chlamydia antigens. In a C. pneumoniae-infected plaque, such T cells may be activated by local antigen and could contribute to the inflammatory process in the arterial wall through CD40 ligand expression and cytokine secretion.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2000.01294.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:22:44Z","timestamp":1047378164000},"page":"261-269","source":"Crossref","is-referenced-by-count":53,"title":["T lymphocyte lines isolated from atheromatous plaque contain cells capable of responding to<i>Chlamydia<\/i>antigens"],"prefix":"10.1093","volume":"121","author":[{"given":"A J","family":"Curry","sequence":"first","affiliation":[{"name":"Departments of Medicine and Neurosurgery, University of Cambridge, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Portig","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Neurosurgery, University of Cambridge, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J C","family":"Goodall","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Neurosurgery, University of Cambridge, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J","family":"Kirkpatrick","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Neurosurgery, University of Cambridge, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J S H","family":"Gaston","sequence":"additional","affiliation":[{"name":"Departments of Medicine and Neurosurgery, University of Cambridge, Cambridge, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,10,9]]},"reference":[{"key":"2022011221083421200_b1","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1001\/jama.1992.03490010070032","article-title":"Association of prior infection with Chlamydia pneumoniae and 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