{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,4,4]],"date-time":"2024-04-04T22:33:35Z","timestamp":1712270015722},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2000,11,1]],"date-time":"2000-11-01T00:00:00Z","timestamp":973036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,10,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n               <jats:p>Here we describe the presence of IgG antibodies, in the sera of patients presenting with insulin-dependent diabetes mellitus (IDDM), that react in Western blots with a 60-kD protein (Mr 60K) from rat hepatic microsomal extracts. Sera from 60 IDDM patients were screened and 31\u00b76% were positive for the Mr 60K band. This antibody reactivity was indistinguishable in terms of both molecular weight and isoelectric point (pI 5\u00b74) from that described in some patients presenting with autoimmune hepatitis who may also develop IDDM. We hypothesized that the type-2 glucose transporter (Glut-2) that is expressed on both hepatocytes and pancreatic \u03b2 cells could be a putative target for the detected antibodies. A polyclonal antisera to rat Glut-2 used in the liver microsome Western blot identified a 60-kD band superimposable upon that evidenced by IDDM sera. Antisera to Glut-2 successfully inhibited the binding of the patient's IgGs to liver microsomes, further suggesting that the two proteins may be identical. Using protein extracts from a rat insulinoma cell line (RIN) transfected with the human Glut-2 cDNA, further evidence was obtained suggesting that these IDDM IgGs are specific for the human Glut-2 transporter.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2000.01366.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:24:44Z","timestamp":1047378284000},"page":"164-169","source":"Crossref","is-referenced-by-count":6,"title":["Autoantibodies in recent onset type-1 diabetic patients to a Mr 60K microsomal hepatic protein: new evidence for autoantibodies to the type-2 glucose transporter"],"prefix":"10.1093","volume":"122","author":[{"given":"M","family":"Pehuet-Figoni","sequence":"first","affiliation":[{"name":"INSERM U 25, H\u00f4pital Necker, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Alvarez","sequence":"additional","affiliation":[{"name":"H\u00f4pital Sainte Justine, Montr\u00e9al, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J-F","family":"Bach","sequence":"additional","affiliation":[{"name":"INSERM U 25, H\u00f4pital Necker, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Chatenoud","sequence":"additional","affiliation":[{"name":"INSERM U 25, H\u00f4pital Necker, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,10,9]]},"reference":[{"key":"2022011221085964600_b1","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1016\/S0022-3476(86)80880-0","article-title":"Liver disease associated with anti-liver-kidney microsome antibody in children","volume":"108","author":"Maggiore","year":"1986","journal-title":"J Pediatr"},{"key":"2022011221085964600_b2","first-page":"354","article-title":"A new antigen recognized by anti-liver-kidney-microsome antibody [LKMA]","volume":"75","author":"Codoner-Franch","year":"1989","journal-title":"Clin Exp Immunol"},{"key":"2022011221085964600_b3","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1097\/00005176-198911000-00007","article-title":"Clinical and immunological heterogeneity of anti-liver-kidney microsome antibody-positive autoimmune hepatitis in children","volume":"9","author":"Codoner-Franch","year":"1989","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"2022011221085964600_b4","doi-asserted-by":"crossref","first-page":"1279","DOI":"10.1016\/S0140-6736(74)90140-8","article-title":"Islet-cell antibodies in diabetes mellitus with auto-immune polyendocrine deficiency","volume":"30","author":"Botazzo","year":"1974","journal-title":"Lancet"},{"key":"2022011221085964600_b5","doi-asserted-by":"crossref","first-page":"548","DOI":"10.2337\/diab.41.4.548","article-title":"Antibodies to glutamic acid decarboxylase discriminate major types of diabetes mellitus","volume":"41","author":"Rowley","year":"1992","journal-title":"Diabetes"},{"key":"2022011221085964600_b6","first-page":"331","article-title":"Microsomal antibodies in active chronic hepatitis and other disorders","volume":"15","author":"Rizetto","year":"1973","journal-title":"Clin Exp Immunol"},{"key":"2022011221085964600_b7","doi-asserted-by":"crossref","first-page":"816","DOI":"10.1097\/00005176-198811000-00004","article-title":"Detection of endoplasmic reticulum antibody positive auto-immune hepatitis in children using an Elisa technique","volume":"7","author":"Paradis","year":"1988","journal-title":"J Pediatr Gastroenterol Nutr"},{"key":"2022011221085964600_b8","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1111\/j.1365-2265.1976.tb02822.x","article-title":"Measurement of circulating thyroid microsomal antibodies by the tanned red cell haemagglutination technique: its usefulness in the diagnosis of auto-immune thyroid diseases","volume":"5","author":"Amino","year":"1976","journal-title":"Clin Endocrinol [Oxf]"},{"key":"2022011221085964600_b9","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1136\/ard.52.2.110","article-title":"Anti-nuclear antibodies in routine analysis: the relevance of putative clinical association","volume":"52","author":"Swaak","year":"1993","journal-title":"Ann Rheum Dis"},{"key":"2022011221085964600_b10","doi-asserted-by":"crossref","first-page":"4489","DOI":"10.1073\/pnas.83.12.4489","article-title":"High-resolution analysis of the human HLA-DR polymorphism by hybridization with sequence specific oligonucleotide probes","volume":"83","author":"Angelini","year":"1986","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022011221085964600_b11","doi-asserted-by":"crossref","first-page":"212","DOI":"10.1083\/jcb.61.1.201","article-title":"Analytical study of microsomes and isolated subcellular membranes from rat liver. II. Preparation and composition of the microsomal fraction","volume":"61","author":"Amar-Costesec","year":"1974","journal-title":"J Cell Biol"},{"key":"2022011221085964600_b12","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1177\/096368979400300512","article-title":"Aberrant function and long-term survival of mouse \u03b2 cells exposed in vitro to high glucose concentrations","volume":"3","author":"Pehuet-Figoni","year":"1994","journal-title":"Cell Transplant"},{"key":"2022011221085964600_b13","doi-asserted-by":"crossref","first-page":"C847","DOI":"10.1152\/ajpcell.1986.251.6.C847","article-title":"d-glucose transport and concentration in tumoral insulin-producing cells","volume":"251","author":"Giroix","year":"1986","journal-title":"Am J Physiol"},{"key":"2022011221085964600_b14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1042\/bj2960113","article-title":"Insulin secretion, insulin content, and glucose phosphorylation in RINm5F insulinoma cells after transfection with human Glut-2 glucose transporter cDNA","volume":"296","author":"Tiedge","year":"1993","journal-title":"Biochem J"},{"key":"2022011221085964600_b15","doi-asserted-by":"crossref","first-page":"4007","DOI":"10.1016\/S0021-9258(19)41496-8","article-title":"High resolution two-dimensional electrophoresis of proteins","volume":"250","author":"O'farrell","year":"1975","journal-title":"J Biol Chem"},{"key":"2022011221085964600_b16","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gels to nitro-cellulose sheets: procedure and some applications","volume":"76","author":"Towbin","year":"1979","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022011221085964600_b17","doi-asserted-by":"crossref","first-page":"1281","DOI":"10.1073\/pnas.90.4.1281","article-title":"Autoantibodies to the GLUT-2 glucose transporter of beta cells in insulin-dependent diabetes mellitus of recent onset","volume":"90","author":"Inman","year":"1993","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022011221085964600_b18","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/0092-8674(88)90051-7","article-title":"Cloning and functional expression in bacteria of a novel glucose transporter present in liver, intestine, kidney, and beta-pancreatic islet cells","volume":"55","author":"Thorens","year":"1988","journal-title":"Cell"},{"key":"2022011221085964600_b19","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1007\/BF00400994","article-title":"Acute insulin response to intravenous glucose, glucagon and arginine in some subjects at risk for type I [insulin-dependent] diabetes mellitus","volume":"34","author":"Bardet","year":"1991","journal-title":"Diabetologia"},{"key":"2022011221085964600_b20","doi-asserted-by":"crossref","first-page":"8688","DOI":"10.1073\/pnas.86.22.8688","article-title":"Cloning and functional expression of a human pancreatic islet glucose-transporter cDNA","volume":"86","author":"Permutt","year":"1989","journal-title":"Proc Natl Acad Sci USA"},{"key":"2022011221085964600_b21","doi-asserted-by":"crossref","first-page":"516","DOI":"10.2337\/diab.33.6.516","article-title":"Differential sensitivity to b cell secretagogues in \u2018early\u2019 type 1 diabetes mellitus","volume":"33","author":"Ganda","year":"1984","journal-title":"Diabetes"},{"key":"2022011221085964600_b22","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1056\/NEJM199003083221003","article-title":"Inhibition of glucose transport into rat islet cells by immunoglobulins from patients with new-onset insulin-dependent diabetes mellitus","volume":"322","author":"Johnson","year":"1990","journal-title":"N Engl J Med"},{"key":"2022011221085964600_b23","doi-asserted-by":"crossref","first-page":"1615","DOI":"10.1172\/JCI114883","article-title":"Reduced beta-cell glucose transporter in new onset diabetic BB rats","volume":"86","author":"Orci","year":"1990","journal-title":"J Clin Invest"}],"container-title":["Clinical and Experimental Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2249.2000.01366.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2249.2000.01366.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/122\/2\/164\/42134760\/j.1365-2249.2000.01366.x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/122\/2\/164\/42134760\/j.1365-2249.2000.01366.x.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T11:48:33Z","timestamp":1676375313000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cei\/article\/122\/2\/164\/6461525"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11]]},"references-count":23,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2008,10,9]]},"published-print":{"date-parts":[[2008,10,9]]}},"URL":"https:\/\/doi.org\/10.1046\/j.1365-2249.2000.01366.x","relation":{},"ISSN":["1365-2249","0009-9104"],"issn-type":[{"value":"1365-2249","type":"electronic"},{"value":"0009-9104","type":"print"}],"subject":[],"published-other":{"date-parts":[[2000,11]]},"published":{"date-parts":[[2000,11]]}}}