{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T06:46:19Z","timestamp":1767422779854},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2000,11,1]],"date-time":"2000-11-01T00:00:00Z","timestamp":973036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,10,9]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>The acidic ribosomal proteins of the protozoan parasite Leishmania infantum have been described as prominent antigens during both human and canine visceral leishmaniasis. In this study we present data showing that the intraperitoneal administration in BALB\/c mice of the Leishmania LiP2a protein, in the absence of any added adjuvants, elicited a strong humoral response as an indication that the protein is a potent immunogen. Despite the evolutionary conservation of the acidic ribosomal proteins, the antibody response was found to be specific for the Leishmania protein. Another remarkable finding was the observation that the LiP2a protein stimulates the in vitro proliferation of splenocytes from either LiP2a-immunized or naive BALB\/c mice. Since similar proliferative indices were observed in T cell-enriched cultures, it is likely that the LiP2a stimulating activity is due mainly to T lymphocyte expansion. Also, the stimulatory effect was demonstrated to be antigen-specific, since the proliferation was abrogated by the presence of anti-LiP2a antibodies. Interestingly, the LiP2a protein stimulated the production of substantial amounts of IFN-\u03b3 in cultured splenocytes from LiP2a-immunized mice. Our data indicate therefore that the immunostimulatory properties shown by this antigen should be taken into account when developing therapeutic and prophylactic vaccines against leishmaniasis.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2000.01372.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:24:44Z","timestamp":1047378284000},"page":"212-218","source":"Crossref","is-referenced-by-count":12,"title":["The<i>Leishmania infantum<\/i>acidic ribosomal protein LiP2a induces a prominent humoral response<i>in vivo<\/i>and stimulates cell proliferation<i>in vitro<\/i>and interferon-gamma (IFN-<i>\u03b3<\/i>) production by murine splenocytes"],"prefix":"10.1093","volume":"122","author":[{"given":"M","family":"Soto","sequence":"first","affiliation":[{"name":"Centro de Biolog\u00eda Molecular \u2018Severo Ochoa\u2019, Universidad Aut\u00f3noma de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Alonso","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular \u2018Severo Ochoa\u2019, Universidad Aut\u00f3noma de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J M","family":"Requena","sequence":"additional","affiliation":[{"name":"Centro de Biolog\u00eda Molecular \u2018Severo Ochoa\u2019, Universidad Aut\u00f3noma de Madrid, Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,10,9]]},"reference":[{"key":"2022011221090089600_b1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/clinids\/22.1.1","article-title":"Clinical spectrum of leishmaniasis","volume":"22","author":"Pearson","year":"1996","journal-title":"Clin Infect 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