{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,4,22]],"date-time":"2023-04-22T01:40:12Z","timestamp":1682127612608},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2001,8,1]],"date-time":"2001-08-01T00:00:00Z","timestamp":996624000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,20]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Amphipathic variable-region heavy chain 11-mer peptides from monoclonal human IgM antiproteinase-3 antibodies were studied for peripheral blood lymphocyte stimulation in 21 patients with Wegener's granulomatosis (WG) or microscopic polyangiitis (MPA), connective tissue disease controls and normal control subjects. Positive T-cell activation was observed in most experiments with WG patients' lymphocytes using amphipathic VH-region peptides from four different human monoclonal anti-PR3 antibodies. Control peptides of the same length but without amphipathic characteristics along with other amphipathic peptides not derived from monoclonal anti-PR3 sequence were employed as controls. No significant lymphocyte stimulation was observed with normal controls, but positive stimulation with amphipathic VH peptides was also recorded in other connective tissue disease controls mainly patients with rheumatoid arthritis. Amphipathic peptides not derived from anti-PR3 sequence did not stimulate WG lymphocytes. Our findings indicate that lymphocyte reactivity as an element of cell-mediated immunity may be activated by amphipathic VH-region amino acid sequences of autoantibodies which are themselves associated with diseases such as WG.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2001.01482.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:27:52Z","timestamp":1047378472000},"page":"323-331","source":"Crossref","is-referenced-by-count":3,"title":["Amphipathic variable region heavy chain peptides derived from monoclonal human Wegener's anti-PR3 antibodies stimulate lymphocytes from patients with Wegener's granulomatosis and microscopic polyangiitis"],"prefix":"10.1093","volume":"125","author":[{"given":"E","family":"Peen","sequence":"first","affiliation":[{"name":"Division of Rheumatology & Clinical Immunology, Department of Medicine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Malone","sequence":"additional","affiliation":[{"name":"Division of Rheumatology & Clinical Immunology, Department of Medicine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Myers","sequence":"additional","affiliation":[{"name":"Division of Rheumatology & Clinical Immunology, Department of Medicine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"R C","family":"Williams","sequence":"additional","affiliation":[{"name":"Division of Rheumatology & Clinical Immunology, Department of Medicine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A B","family":"Peck","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Florida School of Medicine, Gainesville, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Csernok","sequence":"additional","affiliation":[{"name":"University of L\u00fcbeck, L\u00fcbeck, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W L","family":"Gross","sequence":"additional","affiliation":[{"name":"University of L\u00fcbeck, L\u00fcbeck, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Staud","sequence":"additional","affiliation":[{"name":"Division of Rheumatology & Clinical Immunology, Department of Medicine, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,12,20]]},"reference":[{"key":"2022011306430919600_b1","doi-asserted-by":"crossref","first-page":"1651","DOI":"10.1056\/NEJM198806233182504","article-title":"Anti-neutrophil cytoplasmic autoantibodies with specificity for myeloperoxidase in patients with systemic vasculitis and idiopathic necrotizing and crescentic glomerulonephritis","volume":"318","author":"Falk","year":"1988","journal-title":"N Engl J Med"},{"key":"2022011306430919600_b2","doi-asserted-by":"crossref","first-page":"76","DOI":"10.7326\/0003-4819-98-1-76","article-title":"Wegener's granulomatosis: prospective clinical and therapeutic experience with 85 patients for 21 years","volume":"98","author":"Fauci","year":"1983","journal-title":"Ann Intern Med"},{"key":"2022011306430919600_b3","first-page":"181","article-title":"Vasculitis affecting the Kidney. 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