{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T11:26:38Z","timestamp":1751282798499},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2001,6,1]],"date-time":"2001-06-01T00:00:00Z","timestamp":991353600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,20]]},"abstract":"<jats:title>SUMMARY<\/jats:title><jats:p>Viral antigen-specific T cells are important for virus elimination. We studied the hepatitis B virus (HBV)-specific T cell response using flow cytometry. Three phases of HBV infection were studied: Group A, HBeAg (+) chronic hepatitis; Group B, HBeAb (+) HBV carrier after seroconversion; and Group C, HBsAb (+) phase. Peripheral T cells were incubated with recombinant HB core antigen (HBcAg), and intracytoplasmic cytokines were analysed by flow cytometry. HBcAg-specific CD4 and CD8 T cells were identified in all three groups and the number of IFN-\u03b3-positive T cells was greater than TNF-\u03b1-positive T cells. The frequency of IFN-\u03b3-positive CD4 and CD8 T cells was highest in Group C, compared with Groups A and B. No significant difference in the HBcAg-specific T cell response was observed between Group A and Group B. The HBcAg-specific CD8 T cell response was diminished by CD4 depletion, addition of antibody against human leucocyte antigen (HLA) class I, class II or CD40L. Cytokine-positive CD8 T cells without HBcAg stimulation were present at a high frequency (7 of 13 cases) in Group B, but were rare in other groups. HBcAg-specific T cells can be detected at high frequency by a sensitive flow cytometric analysis, and these cells are important for controlling HBV replication.<\/jats:p>","DOI":"10.1046\/j.1365-2249.2001.01561.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:27:49Z","timestamp":1047378469000},"page":"435-444","source":"Crossref","is-referenced-by-count":10,"title":["High frequency of circulating HBcAg-specific CD8 T cells in hepatitis B infection: a flow cytometric analysis"],"prefix":"10.1093","volume":"124","author":[{"given":"S","family":"Matsumura","sequence":"first","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Shimada","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Okano","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Okamoto","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Suzuki","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Hakoda","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Mizuno","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Higashi","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Tsuji","sequence":"additional","affiliation":[{"name":"First Department of Internal Medicine, Okayama University Medical School, Okayama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2001,12,20]]},"reference":[{"key":"2022011305532986400_b1","doi-asserted-by":"crossref","first-page":"489","DOI":"10.1038\/317489a0","article-title":"The hepatitis B virus","volume":"317","author":"Tiollais","year":"1985","journal-title":"Nature"},{"key":"2022011305532986400_b2","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/S0140-6736(81)90585-7","article-title":"07 men in Taiwan","volume":"21","author":"Beasley","year":"1981","journal-title":"Lancet"},{"key":"2022011305532986400_b3","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1084\/jem.174.6.1565","article-title":"Cytotoxic T lymphocytes recognize an HLA-A2-restricted epitope within the hepatitis B virus nucleocapsid antigen","volume":"174","author":"Penna","year":"1991","journal-title":"J Exp Med"},{"key":"2022011305532986400_b4","doi-asserted-by":"crossref","first-page":"4659","DOI":"10.4049\/jimmunol.150.10.4659","article-title":"HLA A2 restricted cytotoxic T lymphocyte responses to multiple hepatitis B surface antigen epitopes during hepatitis B virus infection","volume":"150","author":"Nayersina","year":"1993","journal-title":"J Immunol"},{"key":"2022011305532986400_b5","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1172\/JCI115590","article-title":"Acute exacerbations of chronic type B hepatitis are accompanied by increased T cell responses to hepatitis B core and e antigens. 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