{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T04:42:06Z","timestamp":1698554526256},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,1,4]],"date-time":"2002-01-04T00:00:00Z","timestamp":1010102400000},"content-version":"vor","delay-in-days":1252,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[1998,8]]},"abstract":"<jats:sec><jats:title>OBJECTIVE<\/jats:title><jats:p>There is abundant histological and physiological evidence that serotonin plays a role in the regulation of LH secretion in rats. Studies in human subjects have been few, but their results include the finding that pulsatile administration of <jats:sc>L<\/jats:sc>\u20105\u2010hydroxytryptophan (5\u2010HTP, the immediate precursor of serotonin) amplifies LH secretion in women in the medium\u2010late follicular phase, and that this effect is not due to 5\u2010HTP directly inducing LH secretion by the pituitary. We have investigated whether 5\u2010HTP amplifies LH secretion by enhancing the response of the pituitary to GnRH.<\/jats:p><\/jats:sec><jats:sec><jats:title>PATIENTS<\/jats:title><jats:p>Seven patients aged 20\u201340 years with hypogonadotrophic hypogonadism (HH) of hypothalamic origin (3 men with Kallmann's syndrome, 2 women without anosmia and with GH deficiency, and 2 women with anorexia nervosa).<\/jats:p><\/jats:sec><jats:sec><jats:title>DESIGN<\/jats:title><jats:p>To prime the pituitary, subcutaneous pulsatile GnRH was administered for 7 days at the rate of one 5\u201320\u2003\u03bcg pulse every 90\u2003min. The day before the investigation, this regimen was replaced by 1.5\u20133\u2003\u03bcg intravenous pulses at the same frequency. On the day of the investigation, 3\u2003ml blood samples were taken every 10\u2003min from 0850 to 19.00\u2003hours. After the first two samples, the intravenous GnRH pulse frequency was increased to one per hour and was maintained at this level throughout the rest of the study. The first 4\u2003h of the study acted as a control phase allowing determination of the pituitary response to GnRH. At 1300\u2003h, 75\u2003mg of the aromatic\u2010<jats:sc>L<\/jats:sc>\u2010amino\u2010acid decarboxylase inhibitor carbidopa was administered orally; carbidopa does not cross the blood\u2013brain barrier, and prevents peripheral conversion of 5\u2010HTP to serotonin. At 1600\u2003h, another 75\u2003mg dose of carbidopa was administered, and administration of 8\u201320\u2003mg pulses of 5\u2010HTP at a rate of one pulse per hour was begun.<\/jats:p><\/jats:sec><jats:sec><jats:title>MEASUREMENTS<\/jats:title><jats:p>LH was determined in triplicate by an immunoradiometric assay (IRMA), and LH pulses identified by means of a program developed in our laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>When pulsatile administration of GnRH was accompanied by administration of carbidopa and 5\u2010HTP, LH pulse amplitude (2.32\u2003\u00b1\u20030.71\u2003IU\/l) did not differ significantly from its value in either the GnRH\u2003+ carbidopa phase (2.58\u2003\u00b1\u20031.12\u2003IU\/l) or the unaccompanied GnRH phase (2.77\u2003\u00b1\u20031.76\u2003IU\/l).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p> <jats:sc>L<\/jats:sc>\u20105\u2010hydroxytryptophan\u2010induced amplification of LH secretion in humans is not due to enhancement of the pituitary response to GnRH. The effect of <jats:sc>L<\/jats:sc>\u20105\u2010hydroxytryptophan must therefore be due to its action on the hypothalamus, where it may be hypothesized that it increases GnRH release.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2265.1998.00501.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:37:36Z","timestamp":1047379056000},"page":"203-207","source":"Crossref","is-referenced-by-count":1,"title":["<scp>L<\/scp>\u20105\u2010hydroxytryptophan does not stimulate LH secretion directly from the pituitary in patients with gonadotrophin releasing hormone deficiency"],"prefix":"10.1111","volume":"49","author":[{"given":"Joaquin","family":"Lado\u2010Abeal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Gra\u00f1a","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos","family":"Rey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose","family":"Cabezas\u2010Cerrato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1,4]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"145","article-title":"Investigaci\u00f3n in vivo e in vitro de la participaci\u00f3n del 5\u2010l\u2010hidroxitript\u00f3fano en la regulaci\u00f3n gonadotropa. 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