{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:44:45Z","timestamp":1761518685971},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,1,4]],"date-time":"2002-01-04T00:00:00Z","timestamp":1010102400000},"content-version":"vor","delay-in-days":1221,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:sec><jats:title>OBJECTIVES<\/jats:title><jats:p>The overnight 8\u2010mg dexamethasone suppression test is often used to differentiate Cushing's disease, due to an oversecretion of ACTH from the pituitary gland, from other kinds of Cushing's syndrome. However, a few patients with ACTH\u2010producing pituitary adenoma show no suppression of plasma cortisol after the administration of 8\u2003mg of dexamethasone. To clarify the relationship between the level of glucocorticoid receptor (GR) in the pituitary adenoma and the sensitivity to dexamethasone in Cushing's disease, we thus examined the levels of GR\u03b1 and GR\u03b2 mRNAs in the pituitary adenomas in six patients who were proven at surgery to have pituitary ACTH\u2010producing adenomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>MATERIALS<\/jats:title><jats:p>Total RNA was extracted from six pituitary adenomas and pituitary tissue adjacent to one of the adenomas, and the mRNA levels of GR\u03b1, GR\u03b2, pro\u2010opiomelanocortin (POMC) and \u03b2\u2010actin in these samples were sampled by quantitative RT\u2013PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The GR\u03b1 mRNA levels in the adenomas from the two patients who showed no response to the 8\u2010mg dexamethasone suppression test were significantly lower than those in the adenomas of four patients who showed suppression. The GR\u03b2 mRNA level was much lower than that of GR\u03b1 mRNA but not significantly different among the six adenomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>These results suggest strongly that decreased expression of GR\u03b1 in pituitary adenomas may be the major reason for the marked insensitivity to the 8\u2010mg dexamethasone suppression test observed in two patients with Cushing's disease.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2265.1998.00520.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:38:26Z","timestamp":1047379106000},"page":"301-306","source":"Crossref","is-referenced-by-count":25,"title":["Low level of glucocorticoid receptor messenger ribonucleic acid in pituitary adenomas manifesting Cushing's disease with resistance to a high dose\u2010dexamethasone suppression 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