{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T01:48:37Z","timestamp":1777340917886,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":723,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:sec><jats:title>OBJECTIVE<\/jats:title><jats:p>The aetiology of enhanced adrenal androgen secretion in polycystic ovary syndrome is poorly understood. Previous reports suggest that enhanced peripheral metabolism of cortisol results in decreased negative feedback suppression of ACTH secretion, either by enhanced inactivation of cortisol by 5\u03b1\u2010reductase or impaired reactivation of cortisol by 11\u03b2\u2013hydroxysteroid dehydrogenase type 1 (11\u03b2\u2013HSD1). Endogenous inhibitors of hepatic 11\u03b2\u2013HSD1 can be extracted from urine. We have tested the hypothesis that these are increased in patients with polycystic ovary syndrome.<\/jats:p><\/jats:sec><jats:sec><jats:title>DESIGN<\/jats:title><jats:p>A case\u2010control study.<\/jats:p><\/jats:sec><jats:sec><jats:title>PATIENTS<\/jats:title><jats:p>57 patients with polycystic ovary syndrome and 27 healthy control women.<\/jats:p><\/jats:sec><jats:sec><jats:title>MEASUREMENTS<\/jats:title><jats:p>Aliquots from 24\u2003h urine samples were extracted with Sep\u2010Paks and incubated with rat liver microsomes in which 11\u03b2\u2013HSD1 activity was quantified by conversion of <jats:sup>3<\/jats:sup>H\u2010corticosterone to <jats:sup>3<\/jats:sup>H\u201011\u2010dehydrocorticosterone.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Inhibition of 11\u03b2\u2013HSD1 activity was not different in extracts from patients compared with controls (40.8\u2003\u00b1\u200318.9 arbitrary units in patients <jats:italic>vs.<\/jats:italic> 42.7\u2003\u00b1\u200316.6 in controls, mean (\u00b1\u2003SEM, <jats:italic>P\u2003<\/jats:italic>&gt;\u20030.60) and did not correlate with ratios of cortisol metabolites in urine or with body mass index.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The altered cortisol metabolism in polycystic ovarian syndrome, which is consistent with impaired 11\u03b2\u2013HSD1 activity, cannot be accounted for by increased production of measurable endogenous inhibitors of this enzyme.<\/jats:p><\/jats:sec>","DOI":"10.1046\/j.1365-2265.2000.00893.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:43:58Z","timestamp":1047379438000},"page":"77-80","source":"Crossref","is-referenced-by-count":18,"title":["Endogenous inhibitors of 11\u03b2\u2013hydroxysteroid dehydrogenase type 1 do not explain abnormal cortisol metabolism in polycystic ovary syndrome"],"prefix":"10.1111","volume":"52","author":[{"given":"Brian R.","family":"Walker","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Rodin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman F.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard N.","family":"Clayton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"crossref","first-page":"18939","DOI":"10.1016\/S0021-9258(19)47248-7","article-title":"Cloning and expression of rat cDNA encoding corticosteroid 11\u03b2\u2010dehydrogenase","volume":"264","author":"Agarwal A.K.","year":"1989","journal-title":"Journal of Biological Chemistry"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"25959","DOI":"10.1016\/S0021-9258(18)47142-6","article-title":"NAD+\u2010dependent isoform of 11\u03b2\u2010hydroxysteroid dehydrogenase. 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