{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T19:11:17Z","timestamp":1778094677874,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,1,12]],"date-time":"2002-01-12T00:00:00Z","timestamp":1010793600000},"content-version":"vor","delay-in-days":256,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Endocrinology"],"published-print":{"date-parts":[[2001,5]]},"abstract":"<jats:p><jats:bold>OBJECTIVE<\/jats:bold> Insulin\u2010like growth factor binding protein\u20104 (IGFBP\u20104) belongs to a family of six structurally related IGF\u2010binding proteins that are involved in the modulation of the biological effects of the IGFs. In order to obtain more insight into the clinical significance and regulation of IGFBP\u20104 <jats:italic>in vivo<\/jats:italic> we determined the levels of this protein by a specific radioimmunoassay in the human circulation under normal and various pathological conditions.<\/jats:p><jats:p><jats:bold>DESIGN AND PATIENTS<\/jats:bold> Selected human biological fluids and plasma samples from 804 normal healthy males and females, ranging from 0 to 78\u2003years of age, were analysed. In addition, plasma samples from patients with several disorders (i.e. hypothyroidism, hyperthyroidism, GH\u2010deficiency, acromegaly, cancer, chronic renal failure corticosteroid\u2010treatment) were investigated.<\/jats:p><jats:p><jats:bold>MEASUREMENTS<\/jats:bold> A specific RIA for hIGFBP\u20104 was developed, using a rabbit polyclonal antibody raised against a synthetic peptide containing amino acids 80\u2013103 of the mature hIGFBP\u20104 sequence.<\/jats:p><jats:p><jats:bold>RESULTS<\/jats:bold> In normal individuals circulating IGFBP\u20104 levels in males did not change with age. For females the values tended to increase slightly in older age. Overall, the mean \u00b1\u200aSD for males and females (189\u2003\u00b1\u200383\u2003\u00b5g\/l and 193\u2003\u00b1\u200372\u2003\u00b5g\/l, respectively) were not different. Normative range values of IGFBP\u20104 correlated weakly with those of IGF\u2010II (<jats:italic>r<\/jats:italic>\u2003=\u20030\u00b731, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001).<\/jats:p><jats:p>Neither hypothyroidism nor hyperthyroidism appeared to influence circulating IGFBP\u20104 levels since the levels were within the normal range. Both GH status and pharmacological doses of glucocorticoids, as employed in various chronic diseases, did not seriously affect plasma IGFBP\u20104 either. Under conditions with increased circulating PTH levels, i.e. dialysed adult patients with chronic renal failure (CRF) and subjects with hyperparathyroidism, a weak positive relationship was noted between the plasma contents of IGFBP\u20104 and PTH. An excess of IGFBP\u20104 was found in plasma of both nondialysed and dialysed prepubertal growth retarded children with chronic renal failure (CRF) (mean SDS: 10\u00b775 and 5\u00b778, respectively). IGFBP\u20104 levels were inversely related to glomerular filtration rate. Similar results were obtained for dialysed adults with CRF. In a group of CRF children who had undergone renal transplantation, circulating IGFBP\u20104 levels were markedly lower (mean SDS: 3\u00b775). There was no evidence for an increased secretion of IGFBP\u20104 in the circulation of most of the cancer patients with solid tumours. Several children with acute lymphoblastic leukaemia, however, showed elevated plasma IGFBP\u20104 levels (mean SDS: 1\u00b727). The presence of IGFBP\u20104 could also be demonstrated in other human biological fluids. The highest amounts were found in amniotic fluid (391\u2013717\u2003\u00b5g\/l) and follicular fluid (249\u2013500\u2003\u00b5g\/l).<\/jats:p><jats:p><jats:bold>CONCLUSIONS\u2003MEASUREMENT<\/jats:bold> of plasma IGFBP\u20104 has been shown so far to be of minor clinical relevance. However, the results indicate that different concentration gradients between plasma and various other body fluids may exist. Therefore, it may well be that certain pathophysiological stimuli induce significant alterations in the local turnover rate of IGFBP\u20104 but that they are not reflected by changes in the circulating levels. The possibility of quantifying IGFBP\u20104 by RIA will facilitate further <jats:italic>in vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic> studies on its regulation and function in humans.<\/jats:p>","DOI":"10.1046\/j.1365-2265.2001.01248.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T10:49:29Z","timestamp":1047379769000},"page":"655-664","source":"Crossref","is-referenced-by-count":30,"title":["Plasma levels of insulin\u2010like growth factor binding protein\u20104 (IGFBP\u20104) under normal and pathological conditions"],"prefix":"10.1111","volume":"54","author":[{"given":"J.","family":"Van Doorn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. J. F. H.","family":"Cornelissen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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