{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T06:59:46Z","timestamp":1765609186879},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,21]],"date-time":"2001-12-21T00:00:00Z","timestamp":1008892800000},"content-version":"vor","delay-in-days":295,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:p>The objective of this study was to assess apoptotic activity in gestational trophoblastic disease (GTD) and its prognostic value in hydatidiform mole (HM).<\/jats:p><jats:p>Expression of the specific caspase cleavage site within cytokeratin 18 was assessed immunohistochemically using the monoclonal antibody M30 CytoDeath in 12 spontaneous abortions, 22 partial and 57 complete HM, eight choriocarcinoma (CCA) and 28 normal placentas. The M30 immunoreactivity occurred predominantly in the syncytiotrophoblasts. A significantly higher M30 index in HM and CCA was found when compared with normal placentas and spontaneous abortions (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). The M30 index of those HM which spontaneously regressed was significantly higher than those HM which developed persistent disease requiring chemotherapy (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001). The M30 index correlated with another apoptotic index previously detected by TdT\u2010mediated dUTP nick\u2010end labelling (TUNEL) (<jats:italic>P<\/jats:italic>\u2003=\u20030.007) and the proliferation index assessed by the Ki67 antigen (<jats:italic>P<\/jats:italic>\u2003=\u20030.034).<\/jats:p><jats:p>We conclude that apoptosis is important in the pathogenesis of GTD. Assessment of apoptotic activity in HM by the M30 index may be considered as an alternative prognostic indicator for predicting the clinical behaviour.<\/jats:p>","DOI":"10.1046\/j.1365-2559.2001.01065.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T00:10:42Z","timestamp":1047427842000},"page":"243-249","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Apoptotic activity in gestational trophoblastic disease correlates with clinical outcome: assessment by the caspase\u2010related M30 CytoDeath antibody"],"prefix":"10.1111","volume":"38","author":[{"given":"P M","family":"Chiu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y S","family":"Ngan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"U S","family":"Khoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A N Y","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,21]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"1153","article-title":"Cell death via apoptosis and its relationship to growth, development and differentiation of both tumour and normal cells","volume":"10","author":"Cotter TG","year":"1990","journal-title":"Anticancer Res."},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00048057"},{"key":"e_1_2_6_4_2","first-page":"480","article-title":"Massive apoptosis in infantile myofibromatosis. 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