{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:40:22Z","timestamp":1791009622360,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,7,24]],"date-time":"2003-07-24T00:00:00Z","timestamp":1059004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2003,8]]},"abstract":"<jats:p><jats:bold>Aims:\u2002<\/jats:bold> To correlate the expression of a series of apoptotic and oncogene markers (including p53, Bcl\u20102, BAX, Bcl\u2010X<jats:sub>L<\/jats:sub>, p21<jats:sup>WAF,1\/CIP1<\/jats:sup>, cyclin D1, HER\u20102\/<jats:italic>neu<\/jats:italic>) in thymic epithelial tumours with histological type, stage and resectability and to determine whether the information on HER\u20102\/<jats:italic>neu<\/jats:italic> would be valuable in identifying patients who are eligible for anti\u2010HER\u20102\/<jats:italic>neu<\/jats:italic> treatment.<\/jats:p><jats:p><jats:bold>Methods and results:\u2002<\/jats:bold> Immunohistochemical stains were performed on 16 cases of non\u2010neoplastic thymus, 63 thymomas and 17 thymic carcinomas. Fluorescence in\u2010situ hybridization (FISH) for HER2 was performed to validate the gene amplification. Eighteen thymomas were positive for p53 and 14 of them were low\u2010expressors, with positive cells below 10%. All thymic carcinomas revealed over\u2010expression of p53 with positive cells either between 10% and 50% or &gt;50%. The expression of p53 correlated with histological type and stage in thymoma. In both thymoma and thymic carcinoma, there was a statistically significant correlation between p53 status and resectability, with low expressors having a higher likelihood of being resectable. Thymic carcinomas, regardless of the histological subtypes, uniformly expressed Bcl\u20102, while thymomas showed no or only weak cytoplasmic immunoreactivity. Most thymomas and thymic carcinomas were negative for Bcl\u2010X<jats:sub>L<\/jats:sub>, p21<jats:sup>WAF,1\/CIP1<\/jats:sup> and cyclin D1. The expression of BAX was inconsistent among different histological types. Nine thymic carcinomas revealed membranous positivity for HER\u20102\/<jats:italic>neu<\/jats:italic>, but no HER2 gene amplification could be demonstrated by FISH in any of the cases.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> p53 and Bcl\u20102 are more implicated in the development of thymic carcinoma than thymoma. The higher level of p53 expression and the strong immunopositive pattern of Bcl\u20102 in thymic carcinomas have potential value in the differential diagnosis and prediction of aggressiveness and resectability. On account of the absence of HER2 amplification, patients would probably not benefit from anti\u2010HER\u20102\/<jats:italic>neu<\/jats:italic> treatment.<\/jats:p>","DOI":"10.1046\/j.1365-2559.2003.01663.x","type":"journal-article","created":{"date-parts":[[2004,12,25]],"date-time":"2004-12-25T02:09:56Z","timestamp":1103940596000},"page":"165-172","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Expression of apoptosis\u2010related markers and HER\u20102\/<i>neu<\/i> in thymic epithelial tumours"],"prefix":"10.1111","volume":"43","author":[{"given":"C\u2010C","family":"Pan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P C\u2010H","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u2010S","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u2010Y","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Chiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,7,24]]},"reference":[{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.48.1.267"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202591"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.16.1.395"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90412-3"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90500-P"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19970315)79:6<1114::AID-CNCR9>3.0.CO;2-F"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00192631"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.8.2659"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-60157-6"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(96)90289-0"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00194697"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1997.561"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19971127)73:5<639::AID-IJC5>3.0.CO;2-Y"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.1997.5730805.x"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/104.4.375"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280050064"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1998.tb01370.x"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.1996.d01-540.x"},{"key":"e_1_2_12_20_2","first-page":"983","article-title":"Apoptosis, bcl\u20102 protein, and Fas antigen in thymic epithelial tumors","volume":"10","author":"Tateyama H","year":"1997","journal-title":"Mod. 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