{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T08:29:34Z","timestamp":1759048174922},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":1211,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[1998,9]]},"abstract":"<jats:p>Apoptosis of synovial cells in rheumatoid arthritis (RA) synovium determined <jats:italic>in vivo<\/jats:italic> is suggested to counteract the overgrowth of synovium. Immunohistological examination has revealed the infiltration of activated CD4<jats:sup>+<\/jats:sup> T\u2003cells, which express Fas ligand (FasL), in RA synovium. The presence of a putative antigen (Ag) of autoimmune disorders in a target organ may induce the activation of specific T\u2003cells in the inflammatory region such as RA synovium. We examined the possible role of CD4<jats:sup>+<\/jats:sup> T\u2003cells activated by synovial cells in a staphylococcal enterotoxin B (SEB)\u2010dependent manner, inducing synovial cell apoptosis. Synovial cells were cultured with or without interferon\u2010\u03b3 (IFN\u2010\u03b3) and further incubated with CD4<jats:sup>+<\/jats:sup> T\u2003cells in the presence of SEB. After the cocultivation, both the cytotoxicity and FasL expression of CD4<jats:sup>+<\/jats:sup> T\u2003cells were investigated. Constitutive Fas expression was detected on both unstimulated and IFN\u2010\u03b3\u2010stimulated synovial cells. CD4<jats:sup>+<\/jats:sup> T\u2003cells did not kill SEB\u2010pulsed unstimulated synovial cells efficiently. In contrast, when CD4<jats:sup>+<\/jats:sup> T\u2003cells were incubated with IFN\u2010\u03b3\u2010stimulated synovial cells with SEB whose human leucocyte antigen (HLA)\u2010DR and \u2010DQ expression was markedly induced, significant cytotoxicity by these cells against synovial cells was detected. The addition of anti\u2010HLA\u2010DR and \u2010DQ monoclonal antibodies (mAbs) or human Fas chimeric protein (hFas\u2010Fc) reduced this cytotoxicity. FasL expression of CD4<jats:sup>+<\/jats:sup> T\u2003cells cocultured with IFN\u2010\u03b3\u2010stimulated synovial cells with SEB was significantly induced. Furthermore, the addition of mAbs against CD54, CD58 and CD106 inhibited both the cytotoxicity and FasL expression of CD4<jats:sup>+<\/jats:sup> T\u2003cells induced by IFN\u2010\u03b3\u2010stimulated synovial cells in the presence of SEB, indicating the importance of costimulatory molecules on synovial cells in activating CD4<jats:sup>+<\/jats:sup> T\u2003cells. Our results suggest that CD4<jats:sup>+<\/jats:sup> T\u2003cells are activated by synovial cells by an SEB\u2010dependent manner and express FasL, inducing Fas\u2010mediated apoptosis of the latter cells. These phenomena may regulate the overgrowth of synovial cells in RA synovium.<\/jats:p>","DOI":"10.1046\/j.1365-2567.1998.00569.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T02:19:43Z","timestamp":1047435583000},"page":"38-46","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["CD4<sup>+<\/sup> T\u2010cell\u2010mediated cytotoxicity against staphylococcal enterotoxin B\u2010pulsed synovial cells"],"prefix":"10.1111","volume":"95","author":[{"family":"KAWAKAMI","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"MATSUOKA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"TSUBOI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"URAYAMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"NAKASHIMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"KAWABE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"KOJI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"AOYAGI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"MAEDA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"EGUCHI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2567.1998.00569.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-2567.1998.00569.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-2567.1998.00569.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,11]],"date-time":"2023-09-11T01:55:38Z","timestamp":1694397338000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-2567.1998.00569.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,9]]}},"alternative-id":["10.1046\/j.1365-2567.1998.00569.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-2567.1998.00569.x","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9]]},"assertion":[{"value":"2001-12-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}