{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T19:54:36Z","timestamp":1760644476442},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":693,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>BALB\/c mice resolve <jats:italic>Leishmania donovani<\/jats:italic> infection in the liver over an 8\u201312\u2010week period. However, after an initial phase of 2\u20134\u2003weeks where increases in parasite load are not readily detectable, parasite numbers in the spleen begin to increase reaching maximum levels at 16\u2003weeks post\u2010infection. Thereafter, parasite replication in the spleen is controlled and BALB\/c mice maintain this residual parasite load in the spleen for many months, without further increase. We evaluated functions of CD11C<jats:sup>+<\/jats:sup> splenic dendritic cells throughout the course of <jats:italic>L. donovani<\/jats:italic> infection in the spleen of BALB\/c mice. Unlike the dendritic cell (DC)\u2010specific antigen DEC\u2010205, CD11C was not up\u2010regulated on macrophages during visceral leishmaniasis. No appreciable impairment of splenic DC functions was observed when this antigen\u2010presenting cell subset was purified from 30\u2010day post\u2010infected mice. Significant impairment in inducing allogeneic mixed lymphocyte reaction (MLR) and presenting <jats:italic>L. donovani<\/jats:italic> antigens or keyhole limpet haemocyanin (KLH) to specific T cells was observed with CD11C<jats:sup>+<\/jats:sup> splenic DC purified from 60\u2010day post\u2010infected mice. Functional impairment of splenic DC at 60\u2003days post\u2010infection correlated with their reduced surface expression of major histocompatibility complex (MHC) class II molecules, impairment of interleukin\u201012 (IL\u201012) production and to their ability to suppress interferon\u2010\u03b3 (IFN\u2010\u03b3) production by <jats:italic>Leishmania<\/jats:italic> antigen\u2010primed T cells. Of interest, the impairment of splenic DC in presenting <jats:italic>Leishmania<\/jats:italic> antigens or KLH to specific T cells was corrected at 120\u2003days post\u2010infection, and correlated with their up\u2010regulation of MHC class II expression, IL\u201012 production, induction of IFN\u2010\u03b3 by <jats:italic>Leishmania<\/jats:italic> antigen\u2010primed T cells and the onset of control over splenic parasite replication <jats:italic>in vivo<\/jats:italic>. These results indicate that functional integrity of DC may be important in controlling <jats:italic>L. donovani<\/jats:italic> infection.<\/jats:p>","DOI":"10.1046\/j.1365-2567.2000.00939.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T02:26:44Z","timestamp":1047436004000},"page":"305-313","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Modulation of CD11C<sup>+<\/sup> splenic dendritic cell functions in murine visceral leishmaniasis: correlation with parasite replication in the spleen"],"prefix":"10.1111","volume":"99","author":[{"given":"A.","family":"Basu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Chakrabarti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Saha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Bandyopadhyay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2567.2000.00939.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2567.2000.00939.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-2567.2000.00939.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T15:26:26Z","timestamp":1698593186000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-2567.2000.00939.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1046\/j.1365-2567.2000.00939.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-2567.2000.00939.x","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]},"assertion":[{"value":"2001-12-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}