{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T09:07:35Z","timestamp":1743325655591},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,21]],"date-time":"2001-12-21T00:00:00Z","timestamp":1008892800000},"content-version":"vor","delay-in-days":323,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The contribution of early splenic B\u2010cell populations to the colonization of the ileal Peyer's patch was investigated following the surgical removal of the spleen in a series of 56\u2010day\u2010old fetal sheep. The fetuses were killed at 140\u2003days of gestation and the ileal Peyer's patch, the distal jejunal lymph node which drains the Peyer's patch, and a peripheral lymph node, the superficial cervical lymph node, were examined. Enzyme and immunohistochemical evaluation concluded that the distribution of B cells, T cells and stromal cells in the ileal Peyer's patch was similar in splenectomized and normal fetal sheep. Thus, the presence of the fetal spleen was not essential for the colonization of the ileal Peyer's patch and other early sites of B\u2010cell accumulation would appear capable of generating the necessary precursor populations. Investigation of B\u2010cell populations in lymph nodes used a combination of terminal deoxynucleotidyl\u2010transferase\u2010mediated deoxyuridine\u2010triphosphate nick\u2010end\u2010labelling (TUNEL) histochemistry and immunofluorescence to determine the average number of apoptotic B cells in the primary follicles of the outer cortex of splenectomized and normal lambs. A significantly increased number of apoptotic B cells was present in the distal jejunal lymph node but not in the superficial cervical lymph node of splenectomized lambs. This finding suggests that splenectomy affected prenatal B\u2010cell development in fetal sheep and raises questions as to the regulation of B\u2010cell lymphopoiesis in a species using a post\u2010rearrangement organ of diversification.<\/jats:p>","DOI":"10.1046\/j.1365-2567.2001.01153.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T02:27:59Z","timestamp":1047436079000},"page":"131-136","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Effect of early fetal splenectomy on prenatal B\u2010cell development in sheep"],"prefix":"10.1111","volume":"102","author":[{"given":"C. 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