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Here we have explored the infectability of the B\u2010cell subsets contained in peripheral blood and tonsils, as distinguished by their surface expression of the immunoglobulin isotypes that help to define na\u00efve and memory pools. First we show that both CD21 and major histocompatibility complex (MHC) class II molecules \u2013 respectively, the major receptor and co\u2010receptor for EBV on B cells \u2013 are expressed at similar levels on blood and tonsillar B cells, irrespective of surface immunoglobulin class, indicating that each of the subsets demonstrate an equal potential, at least for infection. Then, following <jats:italic>in vitro<\/jats:italic> infection of total tonsillar B cells, we found that the relative frequencies of immunoglobulin (Ig)M\u2010, IgG\u2010 and IgA\u2010positive cells containing EBV\u2010encoded Epstein\u2013Barr virus nuclear antigen 5 (EBNA5) protein at 48\u2003hr were similar to those of the starting population. However, IgD expression was uniformly decreased, probably as a consequence of cellular activation. These data indicate that recirculating B cells have both the potential for, and susceptibility to, initial infection by EBV, irrespective of the immunoglobulin isotype expressed.<\/jats:p>","DOI":"10.1046\/j.1365-2567.2003.01601.x","type":"journal-article","created":{"date-parts":[[2004,12,27]],"date-time":"2004-12-27T00:55:57Z","timestamp":1104108957000},"page":"427-430","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["B\u2010lymphocyte subpopulations are equally susceptible to Epstein\u2013Barr virus infection, irrespective of immunoglobulin isotype expression"],"prefix":"10.1111","volume":"108","author":[{"given":"Barbro","family":"Ehlin\u2010Henriksson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Gordon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George","family":"Klein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.14.4510"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.5.1490"},{"key":"e_1_2_6_4_2","doi-asserted-by":"crossref","first-page":"4657","DOI":"10.1128\/jvi.71.6.4657-4662.1997","article-title":"Epstein\u2013Barr virus uses HLA class II as a cofactor for infection of B lymphocytes","volume":"71","author":"Li Q","year":"1997","journal-title":"J 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