{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T21:54:37Z","timestamp":1790459677299,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,7,21]],"date-time":"2004-07-21T00:00:00Z","timestamp":1090368000000},"content-version":"vor","delay-in-days":1632,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:italic>Hermes<\/jats:italic>\n                    transposable element is derived from the house fly,\n                    <jats:italic>Musca domestica<\/jats:italic>\n                    , and can incorporate into the germline of the yellow fever mosquito,\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    . Preliminary Southern analyses indicated that\n                    <jats:italic>Hermes<\/jats:italic>\n                    integrated along with the marker gene into the mosquito genomic DNA. Here we show that\n                    <jats:italic>Hermes<\/jats:italic>\n                    integrations are accompanied by the integration of the donor plasmid as well. In addition, breaks in the donor plasmid DNAs do not occur precisely, or at the end of the terminal inverted repeats, and are accompanied by small deletions in the plasmids. Furthermore, integrations do not cause the typical 8\u2010bp duplications of the target site DNA. No integrations are observed in the absence of a source of\n                    <jats:italic>Hermes<\/jats:italic>\n                    transposase. The\n                    <jats:italic>Hermes<\/jats:italic>\n                    transposase clearly did not catalyse precise cut\u2010and\u2010paste transposition in these transformed lines. It may have integrated the transposon through general recombination or through a partial replicative transposition mechanism. The imprecision of\n                    <jats:italic>Hermes<\/jats:italic>\n                    integration may result from interactions of the transposase with an endogenous\n                    <jats:italic>hAT<\/jats:italic>\n                    \u2010like element in the mosquito genome.\n                  <\/jats:p>","DOI":"10.1046\/j.1365-2583.2000.00153.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T21:10:32Z","timestamp":1047417032000},"page":"11-18","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":50,"title":["Structure of\n                    <i>Hermes<\/i>\n                    integrations in the germline of the yellow fever mosquito,\n                    <i>Aedes aegypti<\/i>"],"prefix":"10.1111","volume":"9","author":[{"given":"N.","family":"Jasinskiene","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. J.","family":"Coates","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,7,21]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.20.9693"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(83)80320-9"},{"key":"e_1_2_5_4_1","first-page":"380","article-title":"White eye, a new sex\u2010linked mutant of Aedes aegypti","volume":"28","author":"Bhalla S.C.","year":"1968","journal-title":"Mosquito News"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3748"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00557-5"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.1997.tb00349.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3743"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.12.6181"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-1535-0_2"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/142.3.907"},{"key":"e_1_2_5_12_1","volume-title":"Molecular Cloning, A Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(97)00018-0"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.4.1933"},{"key":"e_1_2_5_16_1","doi-asserted-by":"crossref","unstructured":"Stark K.R.&James A.A.(1998)Isolation and characterization of the gene encoding a novel factor Xa\u2010directed anticoagulant from the yellow fever mosquito Aedes aegypti. J Biol Chem273:2080220809.","DOI":"10.1074\/jbc.273.33.20802"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01260.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0016672300032699"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(98)00020-4"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2583.2000.00153.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-2583.2000.00153.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:50:14Z","timestamp":1762080614000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-2583.2000.00153.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1046\/j.1365-2583.2000.00153.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-2583.2000.00153.x","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]},"assertion":[{"value":"2004-07-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}