{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T22:58:13Z","timestamp":1790981893430,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2003,11,18]],"date-time":"2003-11-18T00:00:00Z","timestamp":1069113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2003,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Single nucleotide polymorphisms (SNPs) are an abundant source of genetic variation among individual organisms. To assess the usefulness of SNPs for genome analysis in the yellow fever mosquito,\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    , we sequenced 25 nuclear genes in each of three strains and analysed nucleotide diversity. The average frequency of nucleotide variation was 12 SNPs per kilobase, indicating that nucleotide variation in\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    is similar to that in other organisms, including\n                    <jats:italic>Drosophila<\/jats:italic>\n                    and the malaria vector\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    . Transition polymorphisms outnumbered transversion polymorphisms, at a ratio of about 2\u00a0:\u00a01. We examined codon usage and confirmed that mutational bias favours G and C ending codons. Codon bias was most pronounced in highly expressed genes. Nucleotide diversity estimates indicated that substitution rates are positively correlated in coding and non\u2010coding regions. Nucleotide diversity varied from one gene to another. The unequal distribution of SNPs among\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    nuclear genes suggests that single base variations are non\u2010neutral and are subject to selective constraints. Our analysis showed that ubiquitously expressed genes have lower polymorphism rates and are likely under strong purifying selection, whereas tissue specific genes and genes with a putative role in parasite defence exhibit higher levels of polymorphism that may be associated with diversifying selection.\n                  <\/jats:p>","DOI":"10.1046\/j.1365-2583.2003.00449.x","type":"journal-article","created":{"date-parts":[[2003,11,21]],"date-time":"2003-11-21T00:22:58Z","timestamp":1069374178000},"page":"631-639","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":40,"title":["Intraspecific DNA variation in nuclear genes of the mosquito\n                    <i>Aedes aegypti<\/i>"],"prefix":"10.1111","volume":"12","author":[{"given":"I.","family":"Morlais","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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