{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T23:29:38Z","timestamp":1777505378411,"version":"3.51.4"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,1,20]],"date-time":"2004-01-20T00:00:00Z","timestamp":1074556800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p> <jats:italic>Photobacterium damsela<\/jats:italic> subsp. <jats:italic>piscicida<\/jats:italic>, the causative agent of fish pasteurellosis, was grown <jats:italic>in vivo<\/jats:italic>. Bacterial cells and extracellular products (ECPs) were analysed via electrophoresis and immunoblot analysis, using specific sea bass antisera. Growth <jats:italic>in vivo<\/jats:italic> induced the synthesis of unique bacterial cell proteins at &gt;206, 206, 21.3, 18, 7.6 and &lt;7.6\u2003kDa. Sea bass serum raised against live bacterial cells of the pathogen and especially a sea bass serum raised against formalin\u2010inactivated bacterial cells grown in a specific novel medium recognized the novel antigens at &gt;206 (associated with iron sequestration), 21.3, 7.6 and &lt;7.6\u2003kDa, suggesting that the latter medium conserves the synthesis of natural bacterial cell proteins <jats:italic>in vitro<\/jats:italic>. <jats:italic>In vivo<\/jats:italic> growth of the pathogen induced the synthesis of more toxic ECPs in comparison with <jats:italic>in vitro<\/jats:italic> growth and an inverse correlation between total protein concentration in the ECPs and toxicity per unit of protein was observed. Substrate\u2010polyacrylamide electrophoresis revealed the presence of <jats:italic>in vivo<\/jats:italic> synthesized ECPs of the pathogen (proteases) at 175, 132, &lt;79 and 48.3\u2003kDa. Histological examination of tissues isolated from fish injected with these ECPs revealed inflammatory and necrotic lesions in the spleen, liver, head kidney, intestine and heart as soon as 48\u2003h post\u2010introduction of the ECPs.<\/jats:p>","DOI":"10.1046\/j.1365-2761.2003.00513.x","type":"journal-article","created":{"date-parts":[[2004,1,20]],"date-time":"2004-01-20T15:45:52Z","timestamp":1074613552000},"page":"1-13","source":"Crossref","is-referenced-by-count":15,"title":["The effect of <i>in vivo<\/i> growth on the cellular and extracellular components of the marine bacterial pathogen <i>Photobacterium damsela<\/i> subsp. 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