{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:27:14Z","timestamp":1761236834650},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":602,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Microscopy"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>We used secondary ion mass spectrometry to image cellular targets of nitrogen oxides (widespread air pollutants) in pollen grains of birch (<jats:italic>Betula verrucosa<\/jats:italic> Ehrh.) and cockfoot (<jats:italic>Dactylis glomerata<\/jats:italic> L.). The pollen samples were exposed to air supplemented with high doses of <jats:sup>15<\/jats:sup>NO. The pollen grains were then fixed, dehydrated using a newly developed \u2018vapour phase\u2019 preparation method and embedded in LRW resin. Semithin sections were then analysed. Imaging was performed in scanning mode. As usual, the two isotopes <jats:sup>14<\/jats:sup>N and <jats:sup>15<\/jats:sup>N were imaged as<jats:sup> 12<\/jats:sup>C<jats:sup>14<\/jats:sup>N<jats:sup>\u2212<\/jats:sup> and <jats:sup>12<\/jats:sup>C<jats:sup>15<\/jats:sup>N<jats:sup>\u2212<\/jats:sup>, respectively. The isotopic percentages of <jats:sup>15<\/jats:sup>N were quantitatively determined either by image processing or by direct analysis. We show that the preferential areas of NO fixation in the pollen cell are the sporoderm and discrete intracytoplasmic structures that we tentatively describe as globoid\u2010like structures similar to those encountered in seeds.<\/jats:p>","DOI":"10.1046\/j.1365-2818.2000.00690.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T06:30:17Z","timestamp":1047450617000},"page":"108-115","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Secondary ion mass spectrometry imaging of the fixation of <sup>15<\/sup>N\u2010labelled NO in pollen grains"],"prefix":"10.1111","volume":"198","author":[{"family":"Lhuissier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Lefebvre","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Gibouin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Demarty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Thellier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Ripoll","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1986.tb02797.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0335-7457(96)80103-1"},{"key":"e_1_2_6_4_2","first-page":"451","article-title":"A new cold stage for SIMS analysis and imaging of frozen\u2010hydrated biological samples","volume":"17","author":"D\u00e9rue C.","year":"1999","journal-title":"J. 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