{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T11:35:35Z","timestamp":1762256135755,"version":"build-2065373602"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,12,14]],"date-time":"2002-12-14T00:00:00Z","timestamp":1039824000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2002,12]]},"abstract":"\n Abstract<\/jats:bold>\n From the Vero Beach strain of the mosquito\n Aedes (Stegomyia) aegypti<\/jats:italic>\n (L.) (Diptera: Culicidae), substrains were selected for susceptibility (SS) and refractoriness (RR) to the dog heartworm\n Dirofilaria immitis<\/jats:italic>\n (Leidy) (Filarioidea: Onchocercidae). These two lines and their reciprocal F\n 1<\/jats:sub>\n hybrids were analysed for genetic variation at 14 enzyme loci, using polyacrylamide gel electrophoresis. Six of the enzyme loci showed variation (sample size 48 alleles\/locus\/line). Three of these were monomorphic in the refractory line but polymorphic in the susceptible, i.e. aconitase hydratase (\n Acoh<\/jats:italic>\n ), isocitrate dehydrogenase\u20101 (\n Idh\u20101<\/jats:italic>\n ) and phosphoglucomutase (\n Pgm<\/jats:italic>\n ).\n <\/jats:p>\n \n The other three loci, glucose\u20106\u2010phosphate isomerase (\n Gpi<\/jats:italic>\n ), hexokinase\u20101 (\n Hk\u20101<\/jats:italic>\n ) and isocitrate dehydrogenase\u20102 (\n Idh\u20102<\/jats:italic>\n ), were polymorphic in both SS and RR lines and their hybrids. At two loci (\n Hk\u20101<\/jats:italic>\n ,\n Pgm<\/jats:italic>\n ) three alleles were detected, whereas the other polymorphic loci had only two alleles. For\n Hk\u20101<\/jats:italic>\n , the most frequent allele was\n Hk\u20101<\/jats:italic>\n 80<\/jats:sup>\n (0.563) in refractory and\n Hk\u20101<\/jats:italic>\n 100<\/jats:sup>\n in the susceptible (0.521) and F\n 1<\/jats:sub>\n hybrids. For\n Pgm<\/jats:italic>\n the most frequent alleles were\n Pgm<\/jats:italic>\n 125<\/jats:sup>\n in the susceptible line (0.646) and\n Pgm<\/jats:italic>\n 100<\/jats:sup>\n in the F\n 1<\/jats:sub>\n hybrids (0.563 and 0.604) and refractory line (1.000). The mean observed heterozygosity (H\n o<\/jats:sub>\n ), the mean Hardy\u2013Weinberg expected heterozygosity (H\n e<\/jats:sub>\n ) and the mean number of alleles per locus in the refractory line were lower, but not significantly so, than in the susceptible line and their reciprocal F1 hybrids; the proportion of polymorphic loci was significantly lower in the refractory than in the susceptible line and their F1 hybrids. Within both lines all polymorphisms were in Hardy\u2013Weinberg equilibrium, whereas significant departures from predicted frequencies were observed in SS\u2003\u00d7\u2003RR hybrids at four polymorphic loci (\n Acoh, Gpi, Hk\u20101<\/jats:italic>\n ,\n Pgm<\/jats:italic>\n ) and at three polymorphic loci (\n Acoh, Hk\u20101<\/jats:italic>\n ,\n Pgm<\/jats:italic>\n ) in RR\u2003\u00d7\u2003SS hybrids. The average Nei's and modified Rogers' genetic distances between the lines were 0.024 and 0.139, respectively. These electrophoretic data show that the refractory line (putatively lacking\n fi<\/jats:italic>\n allele) can be distinguished from the susceptible line (\u2003\n fi<\/jats:italic>\n \/\n fi<\/jats:italic>\n ) and their hybrids (heterozygous\n fi<\/jats:italic>\n ) by isozyme marker frequencies, but it remains to be seen whether this difference is causal or chance linkage. In any case, this model system of\n Ae. aegypti\/D. immitis<\/jats:italic>\n provides opportunities to better understand and manipulate the molecular biology of filariasis transmission.\n <\/jats:p>","DOI":"10.1046\/j.1365-2915.2002.00399.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T09:45:37Z","timestamp":1047462337000},"page":"424-429","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Isoenzyme variation in\n Aedes aegypti<\/i>\n correlated with\n Dirofilaria immitis<\/i>\n infectability"],"prefix":"10.1111","volume":"16","author":[{"given":"J. K.","family":"Nayar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. W.","family":"Knight","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12,14]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(02)00028-2"},{"volume-title":"Biosys\u20102. A Computer Program for the Analysis of Allelic Variation in Genetics.","year":"1997","author":"Black W.C.I.V.","key":"e_1_2_6_3_1"},{"key":"e_1_2_6_4_1","first-page":"438","volume-title":"The Biology of Disease Vectors","author":"Black W.C.I.V.","year":"1996"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.13.4.651-661.2000"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(90)90044-G"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","DOI":"10.1079\/9780851993744.0000","volume-title":"The Biology of Mosquitoes, Vol. 1. 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Florida Medical Entomology Laboratory","author":"Nayar J.K.","year":"1989"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1910(75)90229-2"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000286"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1079\/9780851994369.0213"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/22.1.94"},{"key":"e_1_2_6_29_1","first-page":"35","article-title":"Electrophoretic techniques for the genetic study of mosquitoes","volume":"39","author":"Steiner W.W.M.","year":"1979","journal-title":"Mosquito News"},{"key":"e_1_2_6_30_1","first-page":"131","article-title":"Studies on the filarial prevalence among dogs and the mosquito vectors in Nagasaki City, Western Japan. 5. On the vector mosquitoes of Dirofilaria immitis in Nagasaki City","volume":"15","author":"Suenaga O.","year":"1973","journal-title":"Tropical Medicine (Nagasaki)"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1980.11687397"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a109497"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.082235599"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(81)90060-2"},{"volume-title":"Evolution and the Genetics of Populations, Vol. 4. 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