{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T03:21:56Z","timestamp":1768447316372,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2002,1,4]],"date-time":"2002-01-04T00:00:00Z","timestamp":1010102400000},"content-version":"vor","delay-in-days":795,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Ecology"],"published-print":{"date-parts":[[1999,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In attempts to establish a convenient and reliable method for field collection and archival preservation of insects and their endosymbiotic microorganisms for molecular analysis, acetone, ethanol, and other organic solvents were tested for DNA preservability of the pea aphid <jats:italic>Acyrthosiphon pisum<\/jats:italic> and its intracellular symbiotic bacterium <jats:italic>Buchnera<\/jats:italic> sp. After 6\u00a0months\u2019 storage, not only the band of high\u2010molecular\u2010size DNA but also the bands of rRNA were well preserved in acetone, ethanol, 2\u2010propanol, diethyl ether and ethyl acetate. Polymerase chain reaction (PCR) assays confirmed that the DNA of both the insects and their symbionts was well preserved in these solvents. In contrast, methanol and chloroform showed poor DNA preservability. When water\u2010containing series of acetone and ethanol were examined for DNA preservability, acetone was apparently more robust against water contamination than ethanol. Considering that most biological materials contain high amounts of water, acetone may be a more recommendable preservative for DNA analysis than ethanol which has been widely used for this purpose. The DNA of various insects could be preserved in acetone at room temperature in good condition for several years. In addition to the DNA of the host insects, the DNA of their endosymbionts, including <jats:italic>Buchnera<\/jats:italic> and other mycetocyte symbionts, <jats:italic>Wolbachia<\/jats:italic>, and gut bacteria, was amplified by PCR after several years of acetone storage. The RNA and protein of the pea aphid and its endosymbiont were also preserved for several years in acetone. After 2\u00a0years\u2019 storage in acetone, proteins of <jats:italic>A. pisum<\/jats:italic> could be analysed by sodium dodecyl sulphate\u2013polyacrylamide gel electrophoresis (SDS\u2013PAGE) and immunoblotting, and the endosymbiotic bacteria were successfully detected by immunohistochemistry and <jats:italic>in situ<\/jats:italic> hybridization on the tissue sections.<\/jats:p>","DOI":"10.1046\/j.1365-294x.1999.00795.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T10:34:14Z","timestamp":1047465254000},"page":"1935-1945","source":"Crossref","is-referenced-by-count":186,"title":["Acetone preservation: a practical technique for molecular analysis"],"prefix":"10.1111","volume":"8","author":[{"given":"Takema","family":"Fukatsu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.1.143-169.1995"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01647289"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1000239108771"},{"key":"e_1_2_6_5_1","first-page":"29","volume-title":"Molecular Systematics","author":"Dessauer HC","year":"1996"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2307\/1221122"},{"key":"e_1_2_6_7_1","first-page":"657","article-title":"Interim and archival preservation of plant specimens in alcohols for DNA studies","volume":"20","author":"Flournoy LE","year":"1996","journal-title":"Biotechniques"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0965-1748(92)90155-8"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00556361"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1303\/aez.33.321"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.10.3599-3606.1998"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","unstructured":"FukatsuT ShimadaM(1999)Molecular characterization ofRickettsiasp. in a bruchid beetleKytorhinus sharpianus.Applied Entomology and Zoology 34 391\u2013397.","DOI":"10.1303\/aez.34.391"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1303\/aez.33.461"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(85)90390-0"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020-1790(90)90064-2"},{"key":"e_1_2_6_16_1","volume-title":"Molecular Systematics","author":"Hillis DM","year":"1996"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00277059"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2787(76)90107-6"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)60637-3"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/362709a0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-1978(93)90012-G"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.2307\/1222632"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90623-3"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1994.0071"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1006\/mpev.1996.0083"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1995.0196"},{"key":"e_1_2_6_27_1","volume-title":"Biodiversity","author":"Wilson EO","year":"1988"}],"container-title":["Molecular Ecology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-294x.1999.00795.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-294x.1999.00795.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T15:55:39Z","timestamp":1698508539000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-294x.1999.00795.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,11]]},"references-count":26,"journal-issue":{"issue":"11","published-print":{"date-parts":[[1999,11]]}},"alternative-id":["10.1046\/j.1365-294x.1999.00795.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-294x.1999.00795.x","archive":["Portico"],"relation":{},"ISSN":["0962-1083","1365-294X"],"issn-type":[{"value":"0962-1083","type":"print"},{"value":"1365-294X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,11]]}}}