{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T21:25:23Z","timestamp":1792185923875,"version":"4.4.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,3,1]],"date-time":"2002-03-01T00:00:00Z","timestamp":1014940800000},"content-version":"vor","delay-in-days":790,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>Infection of the endometrium by <jats:italic>Neisseria gonorrhoeae<\/jats:italic> is a pivotal stage in the development of pelvic inflammatory disease in women. An <jats:italic>ex vivo<\/jats:italic> model of cultures of primary human endometrial cells was developed to study gonococcal\u2013host cell interactions. To facilitate these studies, gonococci were transformed with a hybrid shuttle vector containing the <jats:italic>gfp<\/jats:italic> gene from <jats:italic>Aequoria victoria<\/jats:italic>, encoding the green fluorescent protein (GFP), to produce intrinsically fluorescent bacteria. The model demonstrated that both pili and Opa proteins were important for both mediating gonococcal interactions with endometrial cells and inducing the secretion of pro\u2010inflammatory cytokines and chemokines. Pil<jats:sup>+<\/jats:sup> gonococci showed high levels of adherence and invasion, regardless of Opa expression, which was associated with increased secretion of IL\u20108 chemokine and reduced secretion of IL\u20106 cytokine. Gonococcal challenge also caused increased secretion of TNF\u2010\u03b1 cytokine, but this did not correlate with expression of pili or Opa, suggesting that release of components from non\u2010adherent bacteria may be involved in TNF\u2010\u03b1 induction. Thus, the use of cultured primary endometrial cells, together with gonococci expressing green fluorescent protein, has the potential to extend significantly our knowledge, at the molecular level, of the role of this important human pathogen in the immunobiology of pelvic inflammatory disease.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2000.01694.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:28:23Z","timestamp":1047468503000},"page":"32-43","source":"Crossref","is-referenced-by-count":49,"title":["Interaction of primary human endometrial cells with <i>Neisseria gonorrhoeae<\/i> expressing green fluorescent protein"],"prefix":"10.1111","volume":"35","author":[{"given":"Myron","family":"Christodoulides","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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