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Using fluorescence activated cell sorter (FACS)\u2010based technology, we developed a rapid and powerful selection strategy for the isolation of <jats:italic>S. flexneri<\/jats:italic> mutants that are unable to spread from cell to cell. The majority of mutants identified using this strategy harbour mutations that affect the structure of their lipopolysaccharide or the ability of the bacteria to move intracellularly via actin\u2010based motility; both factors have previously been shown to be essential for cell\u2010to\u2010cell spread. However, using a modified strategy that eliminated both of these types of mutants, we identified several mutants that provide us with evidence that bacterial proteins of the type III secretion system, which are essential for bacterial entry into host cells, also play a role in cell\u2010to\u2010cell spread.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2000.01770.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:29:38Z","timestamp":1047468578000},"page":"974-990","source":"Crossref","is-referenced-by-count":50,"title":["The development of a FACS\u2010based strategy for the isolation of <i>Shigella flexneri<\/i> mutants that are deficient in intercellular 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