{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:35:24Z","timestamp":1791160524531,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,1,18]],"date-time":"2002-01-18T00:00:00Z","timestamp":1011312000000},"content-version":"vor","delay-in-days":566,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:p>One of the most virulent and feared bacterial pathogens is <jats:italic>Yersinia pestis<\/jats:italic>, the aetiologic agent of bubonic plague. Characterization of the O\u2010antigen gene clusters of 21 serotypes of <jats:italic>Yersinia pseudotuberculosis<\/jats:italic> and the cryptic O\u2010antigen gene cluster of <jats:italic>Y. pestis<\/jats:italic> showed that the plague bacillus is most closely related to and has evolved from <jats:italic>Y. pseudotuberculosis<\/jats:italic> serotype O:1b. The nucleotide sequences of both gene clusters (about 20.5\u2003kb each) were determined and compared to identify the differences that caused the silencing of the <jats:italic>Y. pestis<\/jats:italic> gene cluster. At the nucleotide sequence level, the loci were 98.9% identical and, of the 17 biosynthetic genes identified from the O:1b gene cluster, five were inactivated in the <jats:italic>Y. pestis<\/jats:italic> cluster, four by insertions or deletions of one nucleotide and one by a deletion of 62 nucleotides. Apparently, the expression of the O\u2010antigen is not beneficial for the virulence or to the lifestyle of <jats:italic>Y. pestis<\/jats:italic> and, therefore, as one step in the evolution of <jats:italic>Y. pestis<\/jats:italic>, the O\u2010antigen gene cluster was inactivated.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2000.01993.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:32:12Z","timestamp":1047468732000},"page":"316-330","source":"Crossref","is-referenced-by-count":215,"title":["Characterization of the O\u2010antigen gene clusters of <i>Yersinia pseudotuberculosis<\/i> and the cryptic O\u2010antigen gene cluster of <i>Yersinia pestis<\/i> shows that the plague bacillus is most closely related to and has evolved from <i>Y. pseudotuberculosis<\/i> serotype O:1b"],"prefix":"10.1111","volume":"37","author":[{"given":"Mikael","family":"Skurnik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Peippo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elise","family":"Ervel\u00e4","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,1,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.24.14043"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1128\/iai.60.3.870-875.1992","article-title":"Lipopolysaccharide O side chain of Yersinia enterocolitica O:3 is an essential virulence factor in an orally infected murine model","volume":"60","author":"Al\u2010Hendy A.","year":"1992","journal-title":"Infect Immun"},{"key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1093\/genetics\/39.4.440","article-title":"Segregation of new lysogenic types during growth of doubly lysogenic strain derived from Escherichia coli K12","volume":"39","author":"Appleyard R.K.","year":"1954","journal-title":"Genetics"},{"key":"e_1_2_6_5_1","volume-title":"Current Protocols in Molecular Biology.","author":"Ausubel F.M.","year":"1987"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02605861"},{"key":"e_1_2_6_7_1","first-page":"1715","volume-title":"Escherichia coli and Salmonella: Cellular and Molecular Biology.","author":"Berlyn M.K.B.","year":"1996"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2144\/98245bm10"},{"key":"e_1_2_6_9_1","first-page":"38","article-title":"V and W antigens in strains of Pasteurella pseudotuberculosis","volume":"41","author":"Burrows T.W.","year":"1960","journal-title":"Br J Exp Pathol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"1002","DOI":"10.1128\/JB.180.4.1002-1007.1998","article-title":"Relationships among the O\u2010antigen gene clusters of Salmonella enterica groups B, D1, D2, and D3","volume":"180","author":"Curd H.","year":"1998","journal-title":"J Bacteriol"},{"key":"e_1_2_6_11_1","unstructured":"Daniels C.(1999)Characterization of proteins involved inShigella flexneriO\u2010antigen biosynthesis. 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