{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T10:03:25Z","timestamp":1789898605975,"version":"4.0.1"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,1,18]],"date-time":"2002-01-18T00:00:00Z","timestamp":1011312000000},"content-version":"vor","delay-in-days":535,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:p>The protozoan parasite <jats:italic>Entamoeba histolytica<\/jats:italic> causes intestinal inflammation and ulceration. Amoebic trophozoites activate the transcription factor NF\u2010\u03baB in human intestinal epithelial cells, initiating an inflammatory response programme with resultant damage to the intestinal tissue. Amoebic cysteine proteinases have been proposed as important virulence factors for amoebiasis. To test the role of amoebic cysteine proteinases in the pathogenesis of amoebic colitis, human intestinal xenografts in SCID mice were infected with <jats:italic>E. histolytica<\/jats:italic> trophozoites expressing an antisense message to <jats:italic>ehcp5<\/jats:italic>. The cysteine proteinase\u2010deficient amoeba failed to induce intestinal epithelial cell production of the inflammatory cytokines interleukin (IL)\u20101B and IL\u20108, and caused significantly less gut inflammation and damage to the intestinal permeability barrier. The critical role of amoebic cysteine proteinases in human gut inflammation and tissue damage may be explained by our discovery that amoebic cysteine proteinases possess IL\u20101B converting enzyme (ICE) activity. This ICE activity could contribute to intestinal inflammation by activating human pIL\u20101B released by damaged intestinal cells. These results demonstrate for the first time that amoebic cysteine proteinases are a key virulence factor in amoebic colitis, and provide a novel mechanism for their activity.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2000.02037.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:32:10Z","timestamp":1047468730000},"page":"542-548","source":"Crossref","is-referenced-by-count":120,"title":["<i>Entamoeba histolytica<\/i> cysteine proteinases with interleukin\u20101 beta converting enzyme (ICE) activity cause intestinal inflammation and tissue damage in amoebiasis"],"prefix":"10.1111","volume":"37","author":[{"given":"Zhi","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Le 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