{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T03:25:21Z","timestamp":1791343521916,"version":"4.3.1"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,4,20]],"date-time":"2002-04-20T00:00:00Z","timestamp":1019260800000},"content-version":"vor","delay-in-days":535,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p> <jats:italic>Brucella melitensis<\/jats:italic> 16M is a Gram\u2010negative \u03b1<jats:sub>2<\/jats:sub>\u2010proteobacterium responsible for abortion in goats and for Malta fever in humans. This facultative intracellular pathogen invades into and survives within both professional and non\u2010professional phagocytes. Signature\u2010tagged mutagenesis (STM) was used to identify genes required for the <jats:italic>in vivo<\/jats:italic> pathogenesis of <jats:italic>Brucella<\/jats:italic>. A library of transposon mutants was screened in a murine infection model. Out of 672 mutants screened, 20 were not recovered after a 5\u2003day passage in BALB\/c mice. The attenuation of 18 mutants was confirmed using an <jats:italic>in vivo<\/jats:italic> competition assay against the wild\u2010type strain. The 18 mutants were characterized further for their ability to replicate in murine macrophages and in HeLa cells. The sequences disrupted by the transposon in the mutants have homology to genes coding for proteins of different functional classes: transport, amino acid and DNA metabolism, transcriptional regulation, peptidoglycan synthesis, a chaperone\u2010like protein and proteins of unknown function. The mutants selected in this study provide new insights into the molecular basis of <jats:italic>Brucella<\/jats:italic> virulence.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2000.02150.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:34:09Z","timestamp":1047468849000},"page":"543-551","source":"Crossref","is-referenced-by-count":146,"title":["Identification and characterization of <i>in vivo<\/i> attenuated mutants of <i>Brucella melitensis<\/i>"],"prefix":"10.1111","volume":"38","author":[{"given":"P.","family":"Lestrate","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. \u2010M.","family":"Delrue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Danese","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Didembourg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Taminiau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Mertens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"De Bolle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Tibor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. 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