{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:38:21Z","timestamp":1791038301302,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,1,5]],"date-time":"2002-01-05T00:00:00Z","timestamp":1010188800000},"content-version":"vor","delay-in-days":430,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p>A predicted protein (CT713) with weak sequence similarity to the major outer membrane protein (20.4% identity) in <jats:italic>Chlamydia trachomatis<\/jats:italic> was identified by <jats:italic>Chlamydia<\/jats:italic> genome analysis. We show that this protein is expressed, surface accessible, localized to the chlamydial outer membrane complex and functions as a porin. This protein, PorB, was highly conserved among different serovars, with nearly identical sequences between serovars D, B, C and L2. Sequence comparison between <jats:italic>C. trachomatis<\/jats:italic> and <jats:italic>Chlamydia pneumoniae<\/jats:italic> showed less conservation between species with 59.3% identity. Immunofluorescence staining with monospecific antisera to purified PorB revealed antigen localized within chlamydial inclusions and found throughout the developmental cycle. Antibodies to PorB neutralized infectivity of <jats:italic>C. trachomatis<\/jats:italic> in an <jats:italic>in vitro<\/jats:italic> neutralization assay confirming that PorB is surface exposed. As PorB was found to be in the outer membrane, as well as having weak structural characteristics similar to major outer membrane protein (MOMP) and other porins, a liposome\u2010swelling assay was used to determine whether this protein had pore\u2010forming capabilities. PorB had pore\u2010forming activity and was shown to be different from MOMP porin activity.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2000.02167.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:34:28Z","timestamp":1047468868000},"page":"772-780","source":"Crossref","is-referenced-by-count":54,"title":["Characterization and functional analysis of PorB, a <i>Chlamydia<\/i> porin and neutralizing target"],"prefix":"10.1111","volume":"38","author":[{"given":"Aya","family":"Kubo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard 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