{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T02:56:32Z","timestamp":1792032992758,"version":"4.4.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,1,5]],"date-time":"2002-01-05T00:00:00Z","timestamp":1010188800000},"content-version":"vor","delay-in-days":430,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p> <jats:italic>Legionella pneumophila<\/jats:italic> is a bacterial pathogen that can enter the human lung and grow inside alveolar macrophages. To grow within phagocytic host cells, the bacteria must create a specialized organelle that restricts fusion with lysosomes. Biogenesis of this replicative organelle is controlled by 24 <jats:italic>dot<\/jats:italic> and <jats:italic>icm<\/jats:italic> genes, which encode a type IV\u2010related transport apparatus. To understand how this transporter functions, isogenic <jats:italic>L. pneumophila dot<\/jats:italic> and <jats:italic>icm<\/jats:italic> mutants were characterized, and three distinct phenotypic categories were identified. Our data show that, in addition to genes that encode the core Dot\/Icm transport apparatus, subsets of genes are required for pore formation and modulation of phagosome trafficking. To understand activities required for virulence at a molecular level, we investigated protein\u2013protein interactions. Specific interactions between different Icm proteins were detected by yeast two\u2010hybrid and gel overlay analysis. These data support a model in which the IcmQ\u2013IcmR complex regulates the formation of a translocation channel that delivers proteins into host cells, and the IcmS\u2013IcmW complex is required for export of virulence determinants that modulate phagosome trafficking.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2000.02176.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:34:28Z","timestamp":1047468868000},"page":"719-736","source":"Crossref","is-referenced-by-count":169,"title":["Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for <i>Legionella pneumophila<\/i> intracellular growth"],"prefix":"10.1111","volume":"38","author":[{"given":"J\u00f6rn","family":"Coers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan 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