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As the main reservoir for choline in the host is phosphatidylcholine, we tested whether other choline\u2010containing molecules associated with eukaryotic membranes could provide an alternative source of choline. <jats:italic>H. influenzae<\/jats:italic> was able to use glycerophosphorylcholine (GPC), an abundant degradation product of phospholipids, as efficiently as free choline. Utilization of GPC required <jats:italic>glpQ<\/jats:italic>, which expresses an enzyme with glycerophosphodiester phosphodiesterase activity. In the absence of free choline, this gene was required for adherent <jats:italic>H. influenzae<\/jats:italic> to obtain choline directly from epithelial cells in culture. GlpQ therefore allows choline to be transferred from the host to the bacterial cell surface.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2001.02571.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:39:47Z","timestamp":1047469187000},"page":"1029-1036","source":"Crossref","is-referenced-by-count":62,"title":["The transfer of choline from the host to the bacterial cell surface requires <i>glpQ<\/i> in <i>Haemophilus influenzae<\/i>"],"prefix":"10.1111","volume":"41","author":[{"given":"Xin","family":"Fan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard","family":"Goldfine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Lysenko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey 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