{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:04:00Z","timestamp":1791263040695,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,3,27]],"date-time":"2002-03-27T00:00:00Z","timestamp":1017187200000},"content-version":"vor","delay-in-days":54,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The <jats:italic>shdA<\/jats:italic> gene is the only determinant known to be required for persistence of <jats:italic>Salmonella enterica<\/jats:italic> serotype Typhimurium (<jats:italic>S<\/jats:italic>. Typhimurium) in the murine caecum and for efficient and prolonged shedding of the organism with the faeces. To study the biological activity of the ShdA protein, we examined its expression and binding activity. ShdA was not detected with anti\u2010ShdA antiserum in <jats:italic>S.<\/jats:italic> Typhimurium strain ATCC14028 grown <jats:italic>in vitro<\/jats:italic>, suggesting that this protein is not expressed under standard conditions of bacterial cultivation in the laboratory. However, in mice infected with <jats:italic>S.<\/jats:italic> Typhimurium, an immunofluorescence signal detected with anti\u2010ShdA antiserum co\u2010localized with that generated by anti\u2010O4 antiserum in thin sections from the caecum. Expression of the cloned <jats:italic>shdA<\/jats:italic> gene from the T7 promoter <jats:italic>in vitro<\/jats:italic> resulted in detection of ShdA in the outer membrane of <jats:italic>S.<\/jats:italic> Typhimurium and in binding of fibronectin to the bacterial surface. Binding of purified glutathione\u2010<jats:italic>S<\/jats:italic>\u2010transferase (GST)\u2013ShdA fusion protein to fibronectin was dose dependent and could be partially inhibited by preincubation with antifibronectin antibodies. GST\u2013ShdA bound to connective tissue and the basement membrane in thin sections from the murine caecum <jats:italic>in situ<\/jats:italic>. A similar labelling pattern was produced when thin sections of the murine caecum were stained with antifibronectin antiserum. Collectively, these data demonstrate that ShdA is a surface\u2010localized, fibronectin\u2010binding protein whose expression is induced <jats:italic>in vivo<\/jats:italic> in the murine caecum, a tissue in which a cognate receptor of this outer membrane protein is expressed.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2002.02805.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T11:42:12Z","timestamp":1047469332000},"page":"895-905","source":"Crossref","is-referenced-by-count":105,"title":["<i>Salmonella enterica<\/i> serotype Typhimurium ShdA is an outer membrane fibronectin\u2010binding protein that is expressed in the intestine"],"prefix":"10.1111","volume":"43","author":[{"given":"Robert A.","family":"Kingsley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato L.","family":"Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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