{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T08:59:22Z","timestamp":1779181162649,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,3,25]],"date-time":"2003-03-25T00:00:00Z","timestamp":1048550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    BAD1, an adhesin and immune modulator of\n                    <jats:italic>Blastomyces dermatitidis<\/jats:italic>\n                    , is an essential virulence factor that is released extracellularly before association with the yeast surface. Here, deletion of the C\u2010terminal EGF\u2010like domain profoundly affected BAD1 function, leading to non\u2010association with yeast, extracellular accumulation and impaired yeast adherence to macrophages. In equilibrium binding assays, \u0394C\u2010term BAD1, lacking an EGF\u2010like domain, bound poorly to BAD1 null yeast, yielding a low affinity (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    , 3\u2003\u00d7\u200310\n                    <jats:sup>\u22127<\/jats:sup>\n                    \u2003M versus 5\u2003\u00d7\u200310\n                    <jats:sup>\u22128<\/jats:sup>\n                    \u2003M) and\n                    <jats:italic>B<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    (1.9\u2003\u00d7\u200310\n                    <jats:sup>5<\/jats:sup>\n                    versus 7.9\u2003\u00d7\u200310\n                    <jats:sup>5<\/jats:sup>\n                    ) compared with BAD1. Similar protein binding profiles were observed using chitin particles, reinforcing the notion that chitin fibrils are a receptor for BAD1, and that the EGF\u2010like domain is critical for BAD1 interactions with chitin on yeast. \u0394C\u2010term strains bound poorly to macrophages, compared with parental or BAD1\u2010reconstituted null strains. However, \u0394C\u2010term strains and the purified protein itself sharply suppressed tumour necrosis factor (TNF)\u2010\u03b1 release by phagocytes\n                    <jats:italic>in vitro<\/jats:italic>\n                    and in lung\n                    <jats:italic>in vivo<\/jats:italic>\n                    , and the strains retained pathogenicity in a murine model of blastomycosis. Our results illustrate the previously undefined role of the EGF\u2010like domain for BAD1 localization to yeast surfaces during cell wall biogenesis. They also demonstrate that the requirements for host cell binding and immune modulation by BAD1 can be dissociated from one another, and that the former is unexpectedly dispensable in the requisite role of BAD1 in pathogenesis.\n                  <\/jats:p>","DOI":"10.1046\/j.1365-2958.2003.03415.x","type":"journal-article","created":{"date-parts":[[2004,12,27]],"date-time":"2004-12-27T17:40:54Z","timestamp":1104169254000},"page":"53-65","source":"Crossref","is-referenced-by-count":82,"title":["A C\u2010terminal EGF\u2010like domain governs BAD1 localization to the yeast surface and fungal adherence to phagocytes, but is dispensable in immune modulation and pathogenicity of\n                    <i>Blastomyces dermatitidis<\/i>"],"prefix":"10.1111","volume":"48","author":[{"given":"Tristan","family":"Brandhorst","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcel","family":"W\u00fcthrich","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Finkel\u2010Jimenez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,3,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"2357","DOI":"10.1242\/jcs.115.11.2357","article-title":"Trimeric assembly of the C\u2010terminal region of Thromobospondin\u20101 or Thrombospondin\u20102 is necessary for cell spreading and fascin spike organisation","volume":"115","author":"Anilkumar N.","year":"2002","journal-title":"J Cell Sci"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(88)80690-2"},{"key":"e_1_2_6_4_1","unstructured":"Aslett M.(2002) Parasite Genomes WU\u2010Blast2 [WWW document].http:www.ebi.ac.ukblast2parasites.html."},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/prep.1997.0783"},{"key":"e_1_2_6_6_1","first-page":"893","article-title":"Molecular evolution of the fungi: human pathogens","volume":"9","author":"Bowman B.H.","year":"1992","journal-title":"Mol Biol Evol"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.11.7925"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.8.1207"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.4.1111"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3306-1_4"},{"key":"e_1_2_6_11_1","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1128\/mr.53.1.148-170.1989","article-title":"Transformation in fungi","volume":"53","author":"Fincham J.R.","year":"1989","journal-title":"Microbiol Rev"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.4.2665"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.11.5746"},{"key":"e_1_2_6_14_1","first-page":"611","volume-title":"Biostatistics: a Methodology for the Health Sciences","author":"Fisher L.D.","year":"1993"},{"key":"e_1_2_6_15_1","doi-asserted-by":"crossref","unstructured":"GenBank(2002) [WWW document]http:www.ncbi.nlm.nih.govGenbankGenbankSearch.html.","DOI":"10.1145\/504689.504690"},{"key":"e_1_2_6_16_1","first-page":"337","volume-title":"82nd Annual Meeting of the American Society for Microbiology 1982.","author":"Green J.H.","year":"1982"},{"key":"e_1_2_6_17_1","first-page":"695","article-title":"Mouse model of pulmonary blastomycosis: utility, simplicity, and quantitative parameters","volume":"117","author":"Harvey R.P.","year":"1978","journal-title":"Am Rev Respir Dis"},{"key":"e_1_2_6_18_1","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1128\/iai.27.3.714-720.1980","article-title":"Alteration of polymorphonuclear leukocytes activity by viable Candida albicans","volume":"27","author":"Hilger A.E.","year":"1980","journal-title":"Infect Immun"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(96)00713-5"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.51.30725"},{"key":"e_1_2_6_21_1","unstructured":"Keath E.J. Hof J. andIgnatov A.(2001)Two step disruption of the yps\u20103 gene inHistoplasma capsulatumstrain G217B.Abstracts of the 101st General Meeting of the American Society for Microbiology Orlando FL May 2001."},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"3890","DOI":"10.1128\/iai.62.9.3890-3900.1994","article-title":"Purification and characterization of the major antigen WI\u20101 from Blastomyces dermatitidis yeasts, and immunological comparison with A antigen","volume":"62","author":"Klein B.S.","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198602273140901"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116571"},{"key":"e_1_2_6_25_1","doi-asserted-by":"crossref","first-page":"3536","DOI":"10.1128\/iai.62.8.3536-3542.1994","article-title":"Altered expression of the surface protein WI\u20101 in genetically related strains of Blastomyces dermatitidis that differ in virulence regulates recognition of yeasts by human macrophages","volume":"62","author":"Klein B.S.","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00613a026"},{"key":"e_1_2_6_27_1","first-page":"719","article-title":"Statistical aspects of the analysis of data from retrospective studies of disease","volume":"22","author":"Mantel N.","year":"1959","journal-title":"J Natl Cancer Inst"},{"key":"e_1_2_6_28_1","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1093\/genetics\/139.4.1533","article-title":"Ectopic integration of transforming DNA is rare among Neurospora transformants selected for gene replacement","volume":"139","author":"Miao V.P.","year":"1995","journal-title":"Genetics"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104218200"},{"key":"e_1_2_6_30_1","unstructured":"Mizrachi I. andRiley L.(2002) Microbial Genome Blast Database 9 [WWW document].http:www.ncbi. nlm.nih.govMicrobblastunfinishedgenome.html."},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.3.580"},{"key":"e_1_2_6_32_1","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1128\/iai.63.2.573-579.1995","article-title":"Antibodies to Cryptococcus neoformans glucuronoxylomannan enhance anti\u2010fungal activity of murine macrophages","volume":"63","author":"Mukherjee J.","year":"1995","journal-title":"Infect Immun"},{"key":"e_1_2_6_33_1","doi-asserted-by":"crossref","first-page":"753","DOI":"10.4049\/jimmunol.154.2.753","article-title":"The WI\u20101 antigen of Blastomyces dermatitidis yeast mediates binding to human macrophage CD11b\/CD18 (CR3) and CD14","volume":"154","author":"Newman S.L.","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1515\/BC.1999.159"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.32.19489"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(99)00262-9"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.4148\/1941-4765.1287"},{"key":"e_1_2_6_38_1","first-page":"3.5.7","volume-title":"Current Protocols in Molecular Biology","author":"Tabor S.","year":"1997"},{"key":"e_1_2_6_39_1","doi-asserted-by":"crossref","unstructured":"TIGR: The Institute for Genomic Research(2002) [WWW document].http:www.tigr.org.","DOI":"10.1145\/504689.504690"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.36.22600"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2850325"},{"key":"e_1_2_6_42_1","doi-asserted-by":"crossref","first-page":"3048","DOI":"10.1128\/iai.64.8.3048-3054.1996","article-title":"Localization of a yeast\u2010phase specific gene product to the cell wall of Histoplasma capsulatum","volume":"64","author":"Weaver C.H.","year":"1996","journal-title":"Infect Immun"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.2001.1311"},{"key":"e_1_2_6_44_1","doi-asserted-by":"publisher","DOI":"10.1080\/02681218880000211"},{"key":"e_1_2_6_45_1","doi-asserted-by":"crossref","first-page":"5443","DOI":"10.1128\/IAI.66.11.5443-5449.1998","article-title":"Immunogenicity and protective efficacy of the WI\u20101 adhesin of Blastomyces dermatitidis","volume":"66","author":"W\u00fcthrich M.","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11037"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.7.1507"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2958.2003.03415.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-2958.2003.03415.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T17:00:58Z","timestamp":1697216458000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-2958.2003.03415.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,3,25]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1046\/j.1365-2958.2003.03415.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-2958.2003.03415.x","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1012857.187506","asserted-by":"object"}]},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,3,25]]}}}