{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:37:13Z","timestamp":1792118233456,"version":"4.4.0"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,4,4]],"date-time":"2003-04-04T00:00:00Z","timestamp":1049414400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Archaea share many similarities with eukarya in their information processing pathways and have proven to be a useful model for studies of DNA replication and transcription, but DNA repair pathways are not well understood in archaea. Nucleotide Excision Repair (NER) deals with many bulky DNA lesions and involves over 30 proteins in eukarya. Archaeal NER has not been characterized biochemically, but homologues of the human repair nucleases XPF and XPG have been identified by homology searches. Crenarchaeal XPF proteins have a simplified domain structure, consisting of the C\u2010terminal nuclease domain conserved \u200a\u200ain \u200a\u200aXPF \u200a\u200aand \u200a\u200aMus81 \u200a\u200abut \u200a\u200alacking \u200a\u200athe \u200a\u200aN\u2010terminal \u2018helicase\u2019 domain that is found in eukaryal and euryarchaeal sequences. Unexpectedly, <jats:italic>Sulfolobus<\/jats:italic> XPF is only active in the presence of the sliding clamp PCNA, which is a heterotrimer in this organism. Interactions with two of the three subunits of PCNA are mediated via a C\u2010terminal interaction motif. The PCNA\u2010XPF complex acts as a structure\u2010specific nuclease on a similar range of DNA flap, bubble and junction substrates as the human protein, suggesting a fundamental conservation through billions of years of evolution.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2003.03444.x","type":"journal-article","created":{"date-parts":[[2004,1,23]],"date-time":"2004-01-23T09:52:33Z","timestamp":1074851553000},"page":"361-371","source":"Crossref","is-referenced-by-count":69,"title":["An archaeal XPF repair endonuclease dependent on a heterotrimeric PCNA"],"prefix":"10.1111","volume":"48","author":[{"given":"J. A.","family":"Roberts","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. D.","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. F.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,4,4]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-8777(99)00042-7"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.5.1223"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.23.8758-8766.2000"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00536-0"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(01)00375-6"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvf112"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.42.24638"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","unstructured":"Dionne I. Nookala R.K. Jackson S.P. Doherty A.J. andBell S.D.(2003)A heterotrimeric PCNA in the hyperthermophilic archaeonSulfolobus solfataricus.Mol Cell11:275\u2013282.","DOI":"10.1016\/S1097-2765(02)00824-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.13.2488"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.8.2045"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.21.6559"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.4.872"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01097"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.39.24522"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01729-7"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/371531a0"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004380000241"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.932201"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.77.227"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.16.8873"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000337200"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M003420200"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.14.7835"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.16.2598"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.13.7.768"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.36401"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.21.5796-5798.1998"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.23.4719"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.02009.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(99)01355-9"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.4.914"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/1521-1878(200011)22:11<997::AID-BIES6>3.0.CO;2-#"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0166"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201820200"}],"container-title":["Molecular Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1046%2Fj.1365-2958.2003.03444.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1046\/j.1365-2958.2003.03444.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T21:31:59Z","timestamp":1697232719000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1046\/j.1365-2958.2003.03444.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,4]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,4]]}},"alternative-id":["10.1046\/j.1365-2958.2003.03444.x"],"URL":"https:\/\/doi.org\/10.1046\/j.1365-2958.2003.03444.x","archive":["Portico"],"relation":{},"ISSN":["0950-382X","1365-2958"],"issn-type":[{"value":"0950-382X","type":"print"},{"value":"1365-2958","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,4]]}}}