{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T14:39:56Z","timestamp":1791383996709,"version":"4.3.3"},"reference-count":59,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,4,4]],"date-time":"2003-04-04T00:00:00Z","timestamp":1049414400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2003,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:italic>Salmonella<\/jats:italic>must express and deploy a type III secretion system located in<jats:italic>Salmonella<\/jats:italic>pathogenicity island 2 (SPI\u20102) in order to survive in host phagocytic vacuoles and to cause systemic infection in mouse models of typhoid fever. A genome\u2010wide approach to screening for<jats:italic>Salmonella<\/jats:italic>genes that are transcriptionally co\u2010regulated<jats:italic>in vitro<\/jats:italic>with SPI\u20102 genes was used to identify bacterial loci that might function in a mouse model of systemic disease. Strains with mutations in three SPI\u20102 co\u2010expressed genes were constructed and tested for their ability to cause disease in mice. We found that<jats:italic>virK<\/jats:italic>, a homologue of a<jats:italic>Shigella<\/jats:italic>virulence determinant, and<jats:italic>rcsC<\/jats:italic>, a sensor kinase, are important at late stages of infection. A second<jats:italic>Salmonella<\/jats:italic>gene that has VirK homology,<jats:italic>somA<\/jats:italic>, is also important for systemic infection in mice. We have shown that expression of both<jats:italic>virK<\/jats:italic>and<jats:italic>somA<\/jats:italic>requires the transcription factor PhoP, whereas<jats:italic>rcsC<\/jats:italic>does not. Additionally,<jats:italic>rcsC<\/jats:italic>expression does not require the transcription factor OmpR, but expression of one of the known targets of RcsC, the<jats:italic>yojN rcsB<\/jats:italic>putative operon, does require OmpR.<jats:italic>virK<\/jats:italic>,<jats:italic>somA<\/jats:italic>and<jats:italic>rcsC<\/jats:italic>are expressed in tissue culture macrophages and confer<jats:italic>Salmonella<\/jats:italic>resistance to the cationic peptide polymyxin B. We conclude that<jats:italic>virK<\/jats:italic>,<jats:italic>somA<\/jats:italic>and<jats:italic>rcsC<\/jats:italic>are important for late stages of<jats:italic>Salmonella<\/jats:italic>enteric fever, and that they probably contribute to the remodelling of the bacterial outer membrane in response to the host environment.<\/jats:p>","DOI":"10.1046\/j.1365-2958.2003.03455.x","type":"journal-article","created":{"date-parts":[[2004,1,23]],"date-time":"2004-01-23T09:52:33Z","timestamp":1074851553000},"page":"385-400","source":"Crossref","is-referenced-by-count":144,"title":["<i>virK<\/i>,<i>somA<\/i>and<i>rcsC<\/i>are important for systemic<i>Salmonella enterica<\/i>serovar Typhimurium infection and cationic peptide resistance"],"prefix":"10.1111","volume":"48","author":[{"given":"Corrella S.","family":"Detweiler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise 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