{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T21:23:06Z","timestamp":1789852986079,"version":"4.0.1"},"reference-count":78,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,4,9]],"date-time":"2003-04-09T00:00:00Z","timestamp":1049846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Molecular Microbiology"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Citrobacter rodentium<\/jats:italic>\n                    is used as an\n                    <jats:italic>in vivo<\/jats:italic>\n                    model system for clinically significant enteric pathogens such as enterohaemorrhagic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (EHEC) and enteropathogenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    (EPEC). These pathogens all colonize the lumen side of the host gastrointestinal tract via attaching and effacing (A\/E) lesion formation. In order to identify genes required for the colonization of A\/E\u2010forming pathogens, a library of signature\u2010tagged transposon mutants of\n                    <jats:italic>C. rodentium<\/jats:italic>\n                    was constructed and screened in mice. Of the 576 mutants tested, 14 were attenuated in their ability to colonize the descending colon. Of these, eight mapped to the locus of enterocyte effacement (LEE), which is required for the formation of A\/E lesions, underlying the importance of this mechanism for pathogenesis. Another mutant, P5H2, was found to have a transposon insertion in an open reading frame that has strong similarity to type IV pilus nucleotide\u2010binding proteins. The region flanking the transposon insertion was sequenced, identifying a cluster of 12 genes that encode the first described pilus of\n                    <jats:italic>C. rodentium<\/jats:italic>\n                    (named\n                    <jats:styled-content>c<\/jats:styled-content>\n                    olonization\n                    <jats:styled-content>f<\/jats:styled-content>\n                    actor\n                    <jats:styled-content>\n                      <jats:italic>C<\/jats:italic>\n                    <\/jats:styled-content>\n                    <jats:italic>itrobacter<\/jats:italic>\n                    , CFC). The proteins encoded by\n                    <jats:italic>cfc<\/jats:italic>\n                    genes have identity to proteins of the type IV COF pilus of enterotoxigenic\n                    <jats:italic>E. coli<\/jats:italic>\n                    (ETEC), the toxin co\u2010regulated pilus of\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    and the bundle\u2010forming pilus of EPEC. A non\u2010polar mutation in\n                    <jats:italic>cfcI<\/jats:italic>\n                    , complementation of this strain with wild\u2010type\n                    <jats:italic>cfcI<\/jats:italic>\n                    and complementation of strain P5H2 with wild\u2010type\n                    <jats:italic>cfcH<\/jats:italic>\n                    confirmed that these genes are required for colonization of the gastrointestinal tract by\n                    <jats:italic>C. rodentium<\/jats:italic>\n                    . Thus, CFC provides a convenient model to study type IV pilus\u2010mediated pathogen\u2013host interactions under physiological conditions in the natural colonic environment.\n                  <\/jats:p>","DOI":"10.1046\/j.1365-2958.2003.03470.x","type":"journal-article","created":{"date-parts":[[2004,12,27]],"date-time":"2004-12-27T17:42:29Z","timestamp":1104169349000},"page":"795-809","source":"Crossref","is-referenced-by-count":90,"title":["Identification of a novel type IV pilus gene cluster required for gastrointestinal colonization of\n                    <i>Citrobacter rodentium<\/i>"],"prefix":"10.1111","volume":"48","author":[{"given":"Rosanna","family":"Mundy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Pickard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca 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